Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28
Abstract
The aim of this study was to investigate the effects of centrally applied somatostatin- 28 on morphometric characteristics of the thymus, the thymocyte subpopulations, as well as, on apoptosis and phases of cell cycle in thymocytes. For this purpose, peripubertal male rats were cannulated intracerebroventriculary and treated with repeated, nanomolar concentrations of somatostatin- 28 ( experimental group) or saline ( control group). Animals were sacrificed and their thymuses were used for the analysis of thymocyte subpopulations, cell cycle and apoptosis by flow cytometry and for the evaluation of morphometric parameters by stereological analysis. Our results showed that somatostatin- 28 caused decrease of the thymic mass and volume, as well as total thymocytes number. Stereological analysis revealed volume decrease of thymic cortex and medulla accompanied with cellularity decrease. Somatostatin in the deeper cortex decreased the number of thymocytes, per volume unit, while in outer co...rtex raised their number. A significant increase in the percentage of double-negative and both single-positive thymocyte subpopulations, in parallel with a diminished percentage of double- positive cells was found. The cellularity of double- positive and single-positive thymocyte subpopulations was decreased. Somatostatin-28 treatment augmented the percentage of apoptotic cells, while the percentage of the cells represented in phases of cell cycle was reduced. These results suggest that somatostatin- 28 induce thymus hypotrophy as result of decreasing cortex and medulla volume and cellularity. Changes in the percentage and cellularity of thymocyte subpopulations and numerical density of thymocytes in outer and deeper cortex, indicate that somatostatin- 28 evoked disturbance in transition of double- negative to double- positive thymocytes.
Source:
Histochemical Journal, 2002, 34, 11-12, 573-582Publisher:
- Springer, Dordrecht
DOI: 10.1023/A:1026047600493
ISSN: 0018-2214
PubMed: 14626348
WoS: 000185680200008
Scopus: 2-s2.0-0242383887
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Institution/Community
TorlakTY - JOUR AU - Petrović-Đergović, Danica M. AU - Rakin, Ana AU - Živković, Irena AU - Mićić, Mileva PY - 2002 UR - http://intor.torlakinstitut.com/handle/123456789/155 AB - The aim of this study was to investigate the effects of centrally applied somatostatin- 28 on morphometric characteristics of the thymus, the thymocyte subpopulations, as well as, on apoptosis and phases of cell cycle in thymocytes. For this purpose, peripubertal male rats were cannulated intracerebroventriculary and treated with repeated, nanomolar concentrations of somatostatin- 28 ( experimental group) or saline ( control group). Animals were sacrificed and their thymuses were used for the analysis of thymocyte subpopulations, cell cycle and apoptosis by flow cytometry and for the evaluation of morphometric parameters by stereological analysis. Our results showed that somatostatin- 28 caused decrease of the thymic mass and volume, as well as total thymocytes number. Stereological analysis revealed volume decrease of thymic cortex and medulla accompanied with cellularity decrease. Somatostatin in the deeper cortex decreased the number of thymocytes, per volume unit, while in outer cortex raised their number. A significant increase in the percentage of double-negative and both single-positive thymocyte subpopulations, in parallel with a diminished percentage of double- positive cells was found. The cellularity of double- positive and single-positive thymocyte subpopulations was decreased. Somatostatin-28 treatment augmented the percentage of apoptotic cells, while the percentage of the cells represented in phases of cell cycle was reduced. These results suggest that somatostatin- 28 induce thymus hypotrophy as result of decreasing cortex and medulla volume and cellularity. Changes in the percentage and cellularity of thymocyte subpopulations and numerical density of thymocytes in outer and deeper cortex, indicate that somatostatin- 28 evoked disturbance in transition of double- negative to double- positive thymocytes. PB - Springer, Dordrecht T2 - Histochemical Journal T1 - Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28 EP - 582 IS - 11-12 SP - 573 VL - 34 DO - 10.1023/A:1026047600493 ER -
@article{ author = "Petrović-Đergović, Danica M. and Rakin, Ana and Živković, Irena and Mićić, Mileva", year = "2002", abstract = "The aim of this study was to investigate the effects of centrally applied somatostatin- 28 on morphometric characteristics of the thymus, the thymocyte subpopulations, as well as, on apoptosis and phases of cell cycle in thymocytes. For this purpose, peripubertal male rats were cannulated intracerebroventriculary and treated with repeated, nanomolar concentrations of somatostatin- 28 ( experimental group) or saline ( control group). Animals were sacrificed and their thymuses were used for the analysis of thymocyte subpopulations, cell cycle and apoptosis by flow cytometry and for the evaluation of morphometric parameters by stereological analysis. Our results showed that somatostatin- 28 caused decrease of the thymic mass and volume, as well as total thymocytes number. Stereological analysis revealed volume decrease of thymic cortex and medulla accompanied with cellularity decrease. Somatostatin in the deeper cortex decreased the number of thymocytes, per volume unit, while in outer cortex raised their number. A significant increase in the percentage of double-negative and both single-positive thymocyte subpopulations, in parallel with a diminished percentage of double- positive cells was found. The cellularity of double- positive and single-positive thymocyte subpopulations was decreased. Somatostatin-28 treatment augmented the percentage of apoptotic cells, while the percentage of the cells represented in phases of cell cycle was reduced. These results suggest that somatostatin- 28 induce thymus hypotrophy as result of decreasing cortex and medulla volume and cellularity. Changes in the percentage and cellularity of thymocyte subpopulations and numerical density of thymocytes in outer and deeper cortex, indicate that somatostatin- 28 evoked disturbance in transition of double- negative to double- positive thymocytes.", publisher = "Springer, Dordrecht", journal = "Histochemical Journal", title = "Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28", pages = "582-573", number = "11-12", volume = "34", doi = "10.1023/A:1026047600493" }
Petrović-Đergović, D. M., Rakin, A., Živković, I.,& Mićić, M.. (2002). Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28. in Histochemical Journal Springer, Dordrecht., 34(11-12), 573-582. https://doi.org/10.1023/A:1026047600493
Petrović-Đergović DM, Rakin A, Živković I, Mićić M. Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28. in Histochemical Journal. 2002;34(11-12):573-582. doi:10.1023/A:1026047600493 .
Petrović-Đergović, Danica M., Rakin, Ana, Živković, Irena, Mićić, Mileva, "Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28" in Histochemical Journal, 34, no. 11-12 (2002):573-582, https://doi.org/10.1023/A:1026047600493 . .