Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors
Samo za registrovane korisnike
2000
Autori
Dimitrijević, MirjanaStanojević, Stanislava
Kovačević-Jovanović, Vesna
Miletić, Tatjana
Vujić-Redžić, V.
Radulović, Jelena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenou...s opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.
Ključne reči:
methionine-enkephalin / leucine-enkephalin / opioid receptors, OF1, OP2, OF3 / plaque-forming cell response / central nervous systemIzvor:
Immunopharmacology, 2000, 49, 3, 255-262Izdavač:
- Elsevier, Amsterdam
DOI: 10.1016/S0162-3109(00)00213-7
ISSN: 0162-3109
PubMed: 10996023
WoS: 000089568600004
Scopus: 2-s2.0-0033829010
Institucija/grupa
TorlakTY - JOUR AU - Dimitrijević, Mirjana AU - Stanojević, Stanislava AU - Kovačević-Jovanović, Vesna AU - Miletić, Tatjana AU - Vujić-Redžić, V. AU - Radulović, Jelena PY - 2000 UR - http://intor.torlakinstitut.com/handle/123456789/124 AB - We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved. PB - Elsevier, Amsterdam T2 - Immunopharmacology T1 - Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors EP - 262 IS - 3 SP - 255 VL - 49 DO - 10.1016/S0162-3109(00)00213-7 ER -
@article{ author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić-Redžić, V. and Radulović, Jelena", year = "2000", abstract = "We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "Immunopharmacology", title = "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors", pages = "262-255", number = "3", volume = "49", doi = "10.1016/S0162-3109(00)00213-7" }
Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T., Vujić-Redžić, V.,& Radulović, J.. (2000). Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology Elsevier, Amsterdam., 49(3), 255-262. https://doi.org/10.1016/S0162-3109(00)00213-7
Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Vujić-Redžić V, Radulović J. Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology. 2000;49(3):255-262. doi:10.1016/S0162-3109(00)00213-7 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić-Redžić, V., Radulović, Jelena, "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors" in Immunopharmacology, 49, no. 3 (2000):255-262, https://doi.org/10.1016/S0162-3109(00)00213-7 . .