Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat
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2000
Authors
Vujić-Redžić, V.Dimitrijević, Mirjana
Stanojević, Stanislava
Kovačević-Jovanović, Vesna
Miletić, Tatjana
Radulović, Jelena
Article (Published version)
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Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dos...e that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.
Keywords:
methionine-enkephalin / anti-methionine-enkephalin / antibodies / paw edema / peritoneal macrophages / H2O2 release / behavior / ratSource:
Neuroimmunomodulation, 2000, 8, 2, 70-77Publisher:
- Karger, Basel
DOI: 10.1159/000026455
ISSN: 1021-7401
PubMed: 10965231
WoS: 000089014700003
Scopus: 2-s2.0-0033803297
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TorlakTY - JOUR AU - Vujić-Redžić, V. AU - Dimitrijević, Mirjana AU - Stanojević, Stanislava AU - Kovačević-Jovanović, Vesna AU - Miletić, Tatjana AU - Radulović, Jelena PY - 2000 UR - http://intor.torlakinstitut.com/handle/123456789/115 AB - Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel. PB - Karger, Basel T2 - Neuroimmunomodulation T1 - Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat EP - 77 IS - 2 SP - 70 VL - 8 DO - 10.1159/000026455 ER -
@article{ author = "Vujić-Redžić, V. and Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Radulović, Jelena", year = "2000", abstract = "Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.", publisher = "Karger, Basel", journal = "Neuroimmunomodulation", title = "Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat", pages = "77-70", number = "2", volume = "8", doi = "10.1159/000026455" }
Vujić-Redžić, V., Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T.,& Radulović, J.. (2000). Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat. in Neuroimmunomodulation Karger, Basel., 8(2), 70-77. https://doi.org/10.1159/000026455
Vujić-Redžić V, Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Radulović J. Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat. in Neuroimmunomodulation. 2000;8(2):70-77. doi:10.1159/000026455 .
Vujić-Redžić, V., Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Radulović, Jelena, "Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat" in Neuroimmunomodulation, 8, no. 2 (2000):70-77, https://doi.org/10.1159/000026455 . .