Immunization-induced thymic atrophy as a contributing factor in strain differences in rat susceptibility to EAE
Само за регистроване кориснике
2018
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: It is suggested that impaired thymopoiesis in autoimmune diseases contributes to their perpetuation. To prove this hypothesis, influence of immunization for EAE on thymopoiesis and the putative thymic−dependent changes in the periphery were examined in susceptible (Dark Agouti, DA) and resistant (Albino Oxford, AO) rats. Methods: On the 13th day post−immunization, expression of differentiation/maturation markers of conventional T cells and regulatory CD4+Foxp3+CD25+ cells (nTregs) on thymocytes, their apoptosis and proliferation, frequency of recent thymic emigrants (RTEs) and CD28null cells in CD4+ and CD8+ peripheral blood lymphocytes (PBLs), and thymic expression and circulating levels of cytokines influencing thymus/thymopoiesis were investigated. Results: In rats of both strains increase in proinflammatory−cytokine circulating levels followed by thymic atrophy and changes at multiple thymocyte developmental points, leading to decreased number of the most mature CD4+ ...and CD8+ TCRaβhi thymocytes and frequency of RTEs among PBLs (as in chronobiological aging), was found. This was more prominent in DA rats. Consistently, compared with AO rats, in DA rats were found higher frequencies
of cytolitic CD28null cells (contributing to target tissue damage) among CD4+ PBLs and cytolitic granzyme B+ CD4+ T cells in spinal cord. Additionally, compared with non−immunized controls, DA rats exhibited greater decline in thymic nTreg generation (reflecting diminished thymic IL−7, IL−2 and IL−15 expression) than AO ones. Conclusions: The study suggests that differences in thymopoiesis, and consequently nTreg generation and CD4+CD28null cell frequency in the periphery, contribute to strain differences in EAE clinical presentation.
Извор:
Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, 2018, P.A2.03.06-Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Nacka-Aleksić, M. AU - Stojanović, M. AU - Pilipović, Ivan AU - Kosec, Duško AU - Leposavić, Gordana PY - 2018 UR - http://intor.torlakinstitut.com/handle/123456789/851 AB - Introduction: It is suggested that impaired thymopoiesis in autoimmune diseases contributes to their perpetuation. To prove this hypothesis, influence of immunization for EAE on thymopoiesis and the putative thymic−dependent changes in the periphery were examined in susceptible (Dark Agouti, DA) and resistant (Albino Oxford, AO) rats. Methods: On the 13th day post−immunization, expression of differentiation/maturation markers of conventional T cells and regulatory CD4+Foxp3+CD25+ cells (nTregs) on thymocytes, their apoptosis and proliferation, frequency of recent thymic emigrants (RTEs) and CD28null cells in CD4+ and CD8+ peripheral blood lymphocytes (PBLs), and thymic expression and circulating levels of cytokines influencing thymus/thymopoiesis were investigated. Results: In rats of both strains increase in proinflammatory−cytokine circulating levels followed by thymic atrophy and changes at multiple thymocyte developmental points, leading to decreased number of the most mature CD4+ and CD8+ TCRaβhi thymocytes and frequency of RTEs among PBLs (as in chronobiological aging), was found. This was more prominent in DA rats. Consistently, compared with AO rats, in DA rats were found higher frequencies of cytolitic CD28null cells (contributing to target tissue damage) among CD4+ PBLs and cytolitic granzyme B+ CD4+ T cells in spinal cord. Additionally, compared with non−immunized controls, DA rats exhibited greater decline in thymic nTreg generation (reflecting diminished thymic IL−7, IL−2 and IL−15 expression) than AO ones. Conclusions: The study suggests that differences in thymopoiesis, and consequently nTreg generation and CD4+CD28null cell frequency in the periphery, contribute to strain differences in EAE clinical presentation. C3 - Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands T1 - Immunization-induced thymic atrophy as a contributing factor in strain differences in rat susceptibility to EAE SP - P.A2.03.06 UR - https://hdl.handle.net/21.15107/rcub_intor_851 ER -
@conference{ author = "Nacka-Aleksić, M. and Stojanović, M. and Pilipović, Ivan and Kosec, Duško and Leposavić, Gordana", year = "2018", abstract = "Introduction: It is suggested that impaired thymopoiesis in autoimmune diseases contributes to their perpetuation. To prove this hypothesis, influence of immunization for EAE on thymopoiesis and the putative thymic−dependent changes in the periphery were examined in susceptible (Dark Agouti, DA) and resistant (Albino Oxford, AO) rats. Methods: On the 13th day post−immunization, expression of differentiation/maturation markers of conventional T cells and regulatory CD4+Foxp3+CD25+ cells (nTregs) on thymocytes, their apoptosis and proliferation, frequency of recent thymic emigrants (RTEs) and CD28null cells in CD4+ and CD8+ peripheral blood lymphocytes (PBLs), and thymic expression and circulating levels of cytokines influencing thymus/thymopoiesis were investigated. Results: In rats of both strains increase in proinflammatory−cytokine circulating levels followed by thymic atrophy and changes at multiple thymocyte developmental points, leading to decreased number of the most mature CD4+ and CD8+ TCRaβhi thymocytes and frequency of RTEs among PBLs (as in chronobiological aging), was found. This was more prominent in DA rats. Consistently, compared with AO rats, in DA rats were found higher frequencies of cytolitic CD28null cells (contributing to target tissue damage) among CD4+ PBLs and cytolitic granzyme B+ CD4+ T cells in spinal cord. Additionally, compared with non−immunized controls, DA rats exhibited greater decline in thymic nTreg generation (reflecting diminished thymic IL−7, IL−2 and IL−15 expression) than AO ones. Conclusions: The study suggests that differences in thymopoiesis, and consequently nTreg generation and CD4+CD28null cell frequency in the periphery, contribute to strain differences in EAE clinical presentation.", journal = "Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands", title = "Immunization-induced thymic atrophy as a contributing factor in strain differences in rat susceptibility to EAE", pages = "P.A2.03.06", url = "https://hdl.handle.net/21.15107/rcub_intor_851" }
Nacka-Aleksić, M., Stojanović, M., Pilipović, I., Kosec, D.,& Leposavić, G.. (2018). Immunization-induced thymic atrophy as a contributing factor in strain differences in rat susceptibility to EAE. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, P.A2.03.06. https://hdl.handle.net/21.15107/rcub_intor_851
Nacka-Aleksić M, Stojanović M, Pilipović I, Kosec D, Leposavić G. Immunization-induced thymic atrophy as a contributing factor in strain differences in rat susceptibility to EAE. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands. 2018;:P.A2.03.06. https://hdl.handle.net/21.15107/rcub_intor_851 .
Nacka-Aleksić, M., Stojanović, M., Pilipović, Ivan, Kosec, Duško, Leposavić, Gordana, "Immunization-induced thymic atrophy as a contributing factor in strain differences in rat susceptibility to EAE" in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands (2018):P.A2.03.06, https://hdl.handle.net/21.15107/rcub_intor_851 .