Posttranslaciona regulacija ekspresije RpoS i PsrA gena u pseudomonas putida WCS358 - uloga ClpXP proteaze
Post-translational regulation of the RpoS and PsrA genes in pseudomonas putida WCS358: The role of ClpXP protease
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RpoS i PsrA proteini su ključni transkripcioni regulatori koji se kod pseudomonada aktiviraju u odgovoru na stacionarnu fazu rasta. Cilj ove studije bio je utvrđivanje uloge ClpXP (ATP zavisna serin proteaza) u stabilnosti RpoS i PsrA proteina tokom različitih faza rasta u Pseudomonas putida WCS358. "Western blot" analiza proteinskih ekstrakata P. putida WCS358 i P. putida WCS358 clpX::Km iz rane eksponencijalne, Kasne eksponencijalne i stacionarne faze rasta, sa antitelima na RpoS i PsrA, pokazala je da ClpXP degraduje RpoS i PsrA u ranoj eksponencijalnoj fazi rasta.
The RpoS and PsrA proteins are key transcriptional regulators that are activated in response to the stationary phase of growth in pseudomonads. This study was designed to establish whether ClpXP (ATP-dependent serine protease) regulates levels of RpoS and PsrA in Pseudomonas putida WCS358. Western blot analysis of P. putida WCS358 protein extracts from the early exponentianl, late exponential, and stationary phases of growth with antibodies against RpoS and PsrA revealed that these proteins are degraded by ClpXP in the early exponential phase of growth. The obtained results demonstrate a role for ClpXP protease in post-translational regulation of proteins encoded by the rpoS and psrA genes in Pseudomonas spp.
Кључне речи:
RpoS / PsrA / pseudomonas / post-translational regulationИзвор:
Archives of Biological Sciences, 2008, 60, 1, 1-4Издавач:
- Srpsko biološko društvo, Beograd, i dr.
DOI: 10.2298/ABS0801001J
ISSN: 0354-4664
WoS: 000254813700002
Scopus: 2-s2.0-53549127255
Институција/група
TorlakTY - JOUR AU - Jovčić, Branko AU - Begović, Jelena AU - Lozo, Jelena AU - Topisirović, Ljubiša AU - Kojić, Milan PY - 2008 UR - http://intor.torlakinstitut.com/handle/123456789/818 AB - RpoS i PsrA proteini su ključni transkripcioni regulatori koji se kod pseudomonada aktiviraju u odgovoru na stacionarnu fazu rasta. Cilj ove studije bio je utvrđivanje uloge ClpXP (ATP zavisna serin proteaza) u stabilnosti RpoS i PsrA proteina tokom različitih faza rasta u Pseudomonas putida WCS358. "Western blot" analiza proteinskih ekstrakata P. putida WCS358 i P. putida WCS358 clpX::Km iz rane eksponencijalne, Kasne eksponencijalne i stacionarne faze rasta, sa antitelima na RpoS i PsrA, pokazala je da ClpXP degraduje RpoS i PsrA u ranoj eksponencijalnoj fazi rasta. AB - The RpoS and PsrA proteins are key transcriptional regulators that are activated in response to the stationary phase of growth in pseudomonads. This study was designed to establish whether ClpXP (ATP-dependent serine protease) regulates levels of RpoS and PsrA in Pseudomonas putida WCS358. Western blot analysis of P. putida WCS358 protein extracts from the early exponentianl, late exponential, and stationary phases of growth with antibodies against RpoS and PsrA revealed that these proteins are degraded by ClpXP in the early exponential phase of growth. The obtained results demonstrate a role for ClpXP protease in post-translational regulation of proteins encoded by the rpoS and psrA genes in Pseudomonas spp. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Posttranslaciona regulacija ekspresije RpoS i PsrA gena u pseudomonas putida WCS358 - uloga ClpXP proteaze T1 - Post-translational regulation of the RpoS and PsrA genes in pseudomonas putida WCS358: The role of ClpXP protease EP - 4 IS - 1 SP - 1 VL - 60 DO - 10.2298/ABS0801001J ER -
@article{ author = "Jovčić, Branko and Begović, Jelena and Lozo, Jelena and Topisirović, Ljubiša and Kojić, Milan", year = "2008", abstract = "RpoS i PsrA proteini su ključni transkripcioni regulatori koji se kod pseudomonada aktiviraju u odgovoru na stacionarnu fazu rasta. Cilj ove studije bio je utvrđivanje uloge ClpXP (ATP zavisna serin proteaza) u stabilnosti RpoS i PsrA proteina tokom različitih faza rasta u Pseudomonas putida WCS358. "Western blot" analiza proteinskih ekstrakata P. putida WCS358 i P. putida WCS358 clpX::Km iz rane eksponencijalne, Kasne eksponencijalne i stacionarne faze rasta, sa antitelima na RpoS i PsrA, pokazala je da ClpXP degraduje RpoS i PsrA u ranoj eksponencijalnoj fazi rasta., The RpoS and PsrA proteins are key transcriptional regulators that are activated in response to the stationary phase of growth in pseudomonads. This study was designed to establish whether ClpXP (ATP-dependent serine protease) regulates levels of RpoS and PsrA in Pseudomonas putida WCS358. Western blot analysis of P. putida WCS358 protein extracts from the early exponentianl, late exponential, and stationary phases of growth with antibodies against RpoS and PsrA revealed that these proteins are degraded by ClpXP in the early exponential phase of growth. The obtained results demonstrate a role for ClpXP protease in post-translational regulation of proteins encoded by the rpoS and psrA genes in Pseudomonas spp.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Posttranslaciona regulacija ekspresije RpoS i PsrA gena u pseudomonas putida WCS358 - uloga ClpXP proteaze, Post-translational regulation of the RpoS and PsrA genes in pseudomonas putida WCS358: The role of ClpXP protease", pages = "4-1", number = "1", volume = "60", doi = "10.2298/ABS0801001J" }
Jovčić, B., Begović, J., Lozo, J., Topisirović, L.,& Kojić, M.. (2008). Posttranslaciona regulacija ekspresije RpoS i PsrA gena u pseudomonas putida WCS358 - uloga ClpXP proteaze. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 60(1), 1-4. https://doi.org/10.2298/ABS0801001J
Jovčić B, Begović J, Lozo J, Topisirović L, Kojić M. Posttranslaciona regulacija ekspresije RpoS i PsrA gena u pseudomonas putida WCS358 - uloga ClpXP proteaze. in Archives of Biological Sciences. 2008;60(1):1-4. doi:10.2298/ABS0801001J .
Jovčić, Branko, Begović, Jelena, Lozo, Jelena, Topisirović, Ljubiša, Kojić, Milan, "Posttranslaciona regulacija ekspresije RpoS i PsrA gena u pseudomonas putida WCS358 - uloga ClpXP proteaze" in Archives of Biological Sciences, 60, no. 1 (2008):1-4, https://doi.org/10.2298/ABS0801001J . .