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dc.creatorAgbaba, Danica
dc.creatorMihajlović, A.
dc.creatorBeljanski, P.
dc.creatorŽivanov-Stakić, Dobrila
dc.creatorVladimirov, S.
dc.date.accessioned2021-02-18T10:20:19Z
dc.date.available2021-02-18T10:20:19Z
dc.date.issued1997
dc.identifier.issn0009-5893
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/80
dc.description.abstractTLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.en
dc.publisherSpringer Heidelberg, Heidelberg
dc.rightsrestrictedAccess
dc.sourceChromatographia
dc.subjectthin-layer chromatographyen
dc.subjectcolumn liquid chromatographyen
dc.subjectPPD-tuberculin solutionen
dc.subject8-hydroxyquinoline sulfate-preservativeen
dc.titleDetermination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatographyen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage148
dc.citation.other45: 145-148
dc.citation.spage145
dc.citation.volume45
dc.identifier.doi10.1007/BF02505552
dc.identifier.rcubconv_500
dc.identifier.scopus2-s2.0-0030995068
dc.identifier.wosA1997XA09900024
dc.type.versionpublishedVersion


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