TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.
TY - JOUR
AU - Agbaba, Danica
AU - Mihajlović, A.
AU - Beljanski, P.
AU - Živanov-Stakić, Dobrila
AU - Vladimirov, S.
PY - 1997
UR - http://intor.torlakinstitut.com/handle/123456789/80
AB - TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.
PB - Springer Heidelberg, Heidelberg
T2 - Chromatographia
T1 - Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography
EP - 148
SP - 145
VL - 45
DO - 10.1007/BF02505552
UR - conv_500
ER -
@article{
author = "Agbaba, Danica and Mihajlović, A. and Beljanski, P. and Živanov-Stakić, Dobrila and Vladimirov, S.",
year = "1997",
abstract = "TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography",
pages = "148-145",
volume = "45",
doi = "10.1007/BF02505552",
url = "conv_500"
}
Agbaba, D., Mihajlović, A., Beljanski, P., Živanov-Stakić, D.,& Vladimirov, S.. (1997). Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography. in Chromatographia
Springer Heidelberg, Heidelberg., 45, 145-148.
https://doi.org/10.1007/BF02505552
conv_500
Agbaba D, Mihajlović A, Beljanski P, Živanov-Stakić D, Vladimirov S. Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography. in Chromatographia. 1997;45:145-148.
doi:10.1007/BF02505552
conv_500 .
Agbaba, Danica, Mihajlović, A., Beljanski, P., Živanov-Stakić, Dobrila, Vladimirov, S., "Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography" in Chromatographia, 45 (1997):145-148,
https://doi.org/10.1007/BF02505552 .,
conv_500 .
