Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells
Аутори
Trifunović, SaraSmiljanić, Katarina
Sickmann, Albert
Solari, Fiorella Andrea
Kolarević, Stoimir
Divac Rankov, Aleksandra
Ljujic, Mila
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Although still considered a safer alternative to classical cigarettes, growing body of work points toharmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect ofe-cigarettes needs to be investigated in more detail considering their widespread use.Methods: In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, withand without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cel-lular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performedvia high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry.Results: E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however itnegatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletionin total number of prot...eins and impairment of mitochondrial function in treated cells. The focal adhesion proteinswere upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of post-translational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data areavailable via ProteomeXchange with identifier PXD032071.Conclusions: Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMsalterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.
Кључне речи:
Electronic cigarettes / Proteomics / Lung fibroblast / Metabolic cooperationИзвор:
Respiratory Research, 2022, 23, 191Издавач:
- BMC Springer Nature
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.1186/s12931-022-02102-w
ISSN: 1465-993X
WoS: 00082604740000
Scopus: 2-s2.0-85134238387
Институција/група
TorlakTY - JOUR AU - Trifunović, Sara AU - Smiljanić, Katarina AU - Sickmann, Albert AU - Solari, Fiorella Andrea AU - Kolarević, Stoimir AU - Divac Rankov, Aleksandra AU - Ljujic, Mila PY - 2022 UR - http://intor.torlakinstitut.com/handle/123456789/781 AB - Background: Although still considered a safer alternative to classical cigarettes, growing body of work points toharmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect ofe-cigarettes needs to be investigated in more detail considering their widespread use.Methods: In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, withand without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cel-lular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performedvia high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry.Results: E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however itnegatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletionin total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteinswere upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of post-translational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data areavailable via ProteomeXchange with identifier PXD032071.Conclusions: Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMsalterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact. PB - BMC Springer Nature T2 - Respiratory Research T1 - Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells IS - 191 VL - 23 DO - 10.1186/s12931-022-02102-w ER -
@article{ author = "Trifunović, Sara and Smiljanić, Katarina and Sickmann, Albert and Solari, Fiorella Andrea and Kolarević, Stoimir and Divac Rankov, Aleksandra and Ljujic, Mila", year = "2022", abstract = "Background: Although still considered a safer alternative to classical cigarettes, growing body of work points toharmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect ofe-cigarettes needs to be investigated in more detail considering their widespread use.Methods: In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, withand without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cel-lular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performedvia high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry.Results: E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however itnegatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletionin total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteinswere upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of post-translational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data areavailable via ProteomeXchange with identifier PXD032071.Conclusions: Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMsalterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.", publisher = "BMC Springer Nature", journal = "Respiratory Research", title = "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells", number = "191", volume = "23", doi = "10.1186/s12931-022-02102-w" }
Trifunović, S., Smiljanić, K., Sickmann, A., Solari, F. A., Kolarević, S., Divac Rankov, A.,& Ljujic, M.. (2022). Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. in Respiratory Research BMC Springer Nature., 23(191). https://doi.org/10.1186/s12931-022-02102-w
Trifunović S, Smiljanić K, Sickmann A, Solari FA, Kolarević S, Divac Rankov A, Ljujic M. Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. in Respiratory Research. 2022;23(191). doi:10.1186/s12931-022-02102-w .
Trifunović, Sara, Smiljanić, Katarina, Sickmann, Albert, Solari, Fiorella Andrea, Kolarević, Stoimir, Divac Rankov, Aleksandra, Ljujic, Mila, "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells" in Respiratory Research, 23, no. 191 (2022), https://doi.org/10.1186/s12931-022-02102-w . .