Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia
Само за регистроване кориснике
2015
Аутори
Veljović, KatarinaPopović, Nikola
Terzić-Vidojević, Amarela
Tolinački, Maja
Mihajlović, Sanja
Jovčić, Branko
Kojić, Milan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of... E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.
Кључне речи:
Surface waters / Phylogenetic analysis / PFGE / Antibiotic-resistant enterococciИзвор:
Environmental Monitoring and Assessment, 2015, 187, 9Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1007/s10661-015-4814-x
ISSN: 0167-6369
PubMed: 26314345
WoS: 000360313000053
Scopus: 2-s2.0-84940568901
URI
https://imagine.imgge.bg.ac.rs/handle/123456789/818http://intor.torlakinstitut.com/handle/123456789/736
Институција/група
TorlakTY - JOUR AU - Veljović, Katarina AU - Popović, Nikola AU - Terzić-Vidojević, Amarela AU - Tolinački, Maja AU - Mihajlović, Sanja AU - Jovčić, Branko AU - Kojić, Milan PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/818 UR - http://intor.torlakinstitut.com/handle/123456789/736 AB - Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia. PB - Springer, Dordrecht T2 - Environmental Monitoring and Assessment T1 - Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia IS - 9 VL - 187 DO - 10.1007/s10661-015-4814-x ER -
@article{ author = "Veljović, Katarina and Popović, Nikola and Terzić-Vidojević, Amarela and Tolinački, Maja and Mihajlović, Sanja and Jovčić, Branko and Kojić, Milan", year = "2015", abstract = "Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.", publisher = "Springer, Dordrecht", journal = "Environmental Monitoring and Assessment", title = "Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia", number = "9", volume = "187", doi = "10.1007/s10661-015-4814-x" }
Veljović, K., Popović, N., Terzić-Vidojević, A., Tolinački, M., Mihajlović, S., Jovčić, B.,& Kojić, M.. (2015). Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. in Environmental Monitoring and Assessment Springer, Dordrecht., 187(9). https://doi.org/10.1007/s10661-015-4814-x
Veljović K, Popović N, Terzić-Vidojević A, Tolinački M, Mihajlović S, Jovčić B, Kojić M. Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2015;187(9). doi:10.1007/s10661-015-4814-x .
Veljović, Katarina, Popović, Nikola, Terzić-Vidojević, Amarela, Tolinački, Maja, Mihajlović, Sanja, Jovčić, Branko, Kojić, Milan, "Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia" in Environmental Monitoring and Assessment, 187, no. 9 (2015), https://doi.org/10.1007/s10661-015-4814-x . .