Influence of ageing on sex and strain differences in immune response to inactivated influenza vaccine
Само за регистроване кориснике
2019
Аутори
Petrović, RaisaArsenović-Ranin, Nevena
Bufan, Biljana
Živković, Irena
Prijić, Ivana
Stoiljković, Vera
Leposavić, Gordana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Efficacy of the immune response to vaccine depends on genetic background, sex and
age of the recipient. However, mechanisms underlying this phenomenon have not been
elucidated, yet. The study investigated influence of sex and age on serum IgG response
to seasonal trivalent inactivated split influenza vaccine (TIV) in BALB/c and C57BL/6
mice, and mechanisms underlying this response. Total serum IgG responses to
influenza virus type A strains declined with aging, in a strain-specific manner.
Consequently, strain differences (greater IgG responses in BALB/c mice) observed in
young mice (three-month-old) were abrogated in old (eighteen-month-old) ones.
However, irrespective of strain and age, females developed stronger influenza type Aspecific IgG responses than males. Despite age-related decrease in influenza B-specific
serum IgG response, it was comparable between old BALB/c and C57BL/6 mice. The
strain/sex-specific differences in age-related changes in the magnitudes of IgG
r...esponses to TIV correlated with those in number of germinal centre (GC) B
splenocytes. These differences were related to those in B splenocyte and CD4+
splenocyte proliferation in culture upon restimulation with influenza viruses from TIV.
The magnitudes of IgG responses also correlated to T follicular regulatory (Tfr)/T
follicular helper and Tfr/GC B splenocyte ratios across all groups of mice. Aging,
irrespective of influenza virus-specificity, affected serum IgG2a(c)/IgG1 ratios
(reflecting IFN-γ/IL-4 production level ratio) in male BALB/c and female C57BL/6
mice, respectively. Thus, although in young mice of both strains these ratios were
comparable between sexes, in old females they were shifted towards IgG1 when
compared with age-matched males. Consistently, the IFN-γ/IL-4 production level ratios
in splenocyte cultures stimulated with influenza viruses from old females of both strains
were shifted towards IL-4 compared with that in age-matched male cultures. The study
stimulates further research to formulate sex-specific strategies to improve efficacy of
influenza vaccine in elderly.
Извор:
Immunology at the confluence of multidisciplinary approaches: Abstract book, Hotel Mona Plaza, Belgrade, December 6th-8th, 2019Издавач:
- Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade Immunological Society of Serbia
Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Petrović, Raisa AU - Arsenović-Ranin, Nevena AU - Bufan, Biljana AU - Živković, Irena AU - Prijić, Ivana AU - Stoiljković, Vera AU - Leposavić, Gordana PY - 2019 UR - http://intor.torlakinstitut.com/handle/123456789/692 AB - Efficacy of the immune response to vaccine depends on genetic background, sex and age of the recipient. However, mechanisms underlying this phenomenon have not been elucidated, yet. The study investigated influence of sex and age on serum IgG response to seasonal trivalent inactivated split influenza vaccine (TIV) in BALB/c and C57BL/6 mice, and mechanisms underlying this response. Total serum IgG responses to influenza virus type A strains declined with aging, in a strain-specific manner. Consequently, strain differences (greater IgG responses in BALB/c mice) observed in young mice (three-month-old) were abrogated in old (eighteen-month-old) ones. However, irrespective of strain and age, females developed stronger influenza type Aspecific IgG responses than males. Despite age-related decrease in influenza B-specific serum IgG response, it was comparable between old BALB/c and C57BL/6 mice. The strain/sex-specific differences in age-related changes in the magnitudes of IgG responses to TIV correlated with those in number of germinal centre (GC) B splenocytes. These differences were related to those in B splenocyte and CD4+ splenocyte proliferation in culture upon restimulation with influenza viruses from TIV. The magnitudes of IgG responses also correlated to T follicular regulatory (Tfr)/T follicular helper and Tfr/GC B splenocyte ratios across all groups of mice. Aging, irrespective of influenza virus-specificity, affected serum IgG2a(c)/IgG1 ratios (reflecting IFN-γ/IL-4 production level ratio) in male BALB/c and female C57BL/6 mice, respectively. Thus, although in young mice of both strains these ratios were comparable between sexes, in old females they were shifted towards IgG1 when compared with age-matched males. Consistently, the IFN-γ/IL-4 production level ratios in splenocyte cultures stimulated with influenza viruses from old females of both strains were shifted towards IL-4 compared with that in age-matched male cultures. The study stimulates further research to formulate sex-specific strategies to improve efficacy of influenza vaccine in elderly. PB - Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade Immunological Society of Serbia C3 - Immunology at the confluence of multidisciplinary approaches: Abstract book, Hotel Mona Plaza, Belgrade, December 6th-8th T1 - Influence of ageing on sex and strain differences in immune response to inactivated influenza vaccine UR - https://hdl.handle.net/21.15107/rcub_intor_692 ER -
@conference{ author = "Petrović, Raisa and Arsenović-Ranin, Nevena and Bufan, Biljana and Živković, Irena and Prijić, Ivana and Stoiljković, Vera and Leposavić, Gordana", year = "2019", abstract = "Efficacy of the immune response to vaccine depends on genetic background, sex and age of the recipient. However, mechanisms underlying this phenomenon have not been elucidated, yet. The study investigated influence of sex and age on serum IgG response to seasonal trivalent inactivated split influenza vaccine (TIV) in BALB/c and C57BL/6 mice, and mechanisms underlying this response. Total serum IgG responses to influenza virus type A strains declined with aging, in a strain-specific manner. Consequently, strain differences (greater IgG responses in BALB/c mice) observed in young mice (three-month-old) were abrogated in old (eighteen-month-old) ones. However, irrespective of strain and age, females developed stronger influenza type Aspecific IgG responses than males. Despite age-related decrease in influenza B-specific serum IgG response, it was comparable between old BALB/c and C57BL/6 mice. The strain/sex-specific differences in age-related changes in the magnitudes of IgG responses to TIV correlated with those in number of germinal centre (GC) B splenocytes. These differences were related to those in B splenocyte and CD4+ splenocyte proliferation in culture upon restimulation with influenza viruses from TIV. The magnitudes of IgG responses also correlated to T follicular regulatory (Tfr)/T follicular helper and Tfr/GC B splenocyte ratios across all groups of mice. Aging, irrespective of influenza virus-specificity, affected serum IgG2a(c)/IgG1 ratios (reflecting IFN-γ/IL-4 production level ratio) in male BALB/c and female C57BL/6 mice, respectively. Thus, although in young mice of both strains these ratios were comparable between sexes, in old females they were shifted towards IgG1 when compared with age-matched males. Consistently, the IFN-γ/IL-4 production level ratios in splenocyte cultures stimulated with influenza viruses from old females of both strains were shifted towards IL-4 compared with that in age-matched male cultures. The study stimulates further research to formulate sex-specific strategies to improve efficacy of influenza vaccine in elderly.", publisher = "Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade Immunological Society of Serbia", journal = "Immunology at the confluence of multidisciplinary approaches: Abstract book, Hotel Mona Plaza, Belgrade, December 6th-8th", title = "Influence of ageing on sex and strain differences in immune response to inactivated influenza vaccine", url = "https://hdl.handle.net/21.15107/rcub_intor_692" }
Petrović, R., Arsenović-Ranin, N., Bufan, B., Živković, I., Prijić, I., Stoiljković, V.,& Leposavić, G.. (2019). Influence of ageing on sex and strain differences in immune response to inactivated influenza vaccine. in Immunology at the confluence of multidisciplinary approaches: Abstract book, Hotel Mona Plaza, Belgrade, December 6th-8th Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade Immunological Society of Serbia.. https://hdl.handle.net/21.15107/rcub_intor_692
Petrović R, Arsenović-Ranin N, Bufan B, Živković I, Prijić I, Stoiljković V, Leposavić G. Influence of ageing on sex and strain differences in immune response to inactivated influenza vaccine. in Immunology at the confluence of multidisciplinary approaches: Abstract book, Hotel Mona Plaza, Belgrade, December 6th-8th. 2019;. https://hdl.handle.net/21.15107/rcub_intor_692 .
Petrović, Raisa, Arsenović-Ranin, Nevena, Bufan, Biljana, Živković, Irena, Prijić, Ivana, Stoiljković, Vera, Leposavić, Gordana, "Influence of ageing on sex and strain differences in immune response to inactivated influenza vaccine" in Immunology at the confluence of multidisciplinary approaches: Abstract book, Hotel Mona Plaza, Belgrade, December 6th-8th (2019), https://hdl.handle.net/21.15107/rcub_intor_692 .