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dc.creatorStanojević, Stanislava
dc.creatorBlagojević, Veljko
dc.creatorĆuruvija, Ivana
dc.creatorVujić, Vesna
dc.date.accessioned2021-09-14T10:32:23Z
dc.date.available2021-09-14T10:32:23Z
dc.date.issued2021
dc.identifier.issn0360-3997
dc.identifier.issn1573-2576 (eISSN)
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/611
dc.description.abstractGut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.sr
dc.language.isoensr
dc.publisherSpringer Naturesr
dc.relationThis work was supported by Ministry of Education, Science and Technological Development Republic of Serbia (contract number 451–03-68/2020–14/200177)sr
dc.rightsrestrictedAccesssr
dc.sourceInflammationsr
dc.subjectAlbino Oxford (AO) ratssr
dc.subjectDark Agouti (DA) ratssr
dc.subjectLactobacillus rhamnosussr
dc.subjectPeritoneal cavity immune cellssr
dc.subjectE.colisr
dc.subjectEnterococcus sppsr
dc.titleLactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunitysr
dc.typearticlesr
dc.rights.licenseARRsr
dc.rights.holderSpringer Naturesr
dc.citation.rankM22~
dc.description.otherhttps://rdcu.be/cxynksr
dc.identifier.doi10.1007/s10753-021-01513-z
dc.identifier.scopus2-s2.0-85114681598
dc.identifier.wos000694782600001
dc.type.versionpublishedVersionsr


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