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dc.creatorMilovanović, Vladimir
dc.creatorMinić, Rajna
dc.creatorVakić, J.
dc.creatorIvanović, S.
dc.creatorCupić, V.
dc.creatorBorozan, S.
dc.creatorNešić, A.
dc.creatorŽivković, Irena
dc.date.accessioned2021-02-18T10:54:18Z
dc.date.available2021-02-18T10:54:18Z
dc.date.issued2021
dc.identifier.issn0041-0101
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/572
dc.description.abstractThe MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.en
dc.publisherElsevier Ltd
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200177/RS//
dc.rightsrestrictedAccess
dc.sourceToxicon
dc.subjectAntivenomen
dc.subjectL-Amino acid Oxidase monomeren
dc.subjectMTTen
dc.subjectPotency testen
dc.subjectVipera ammodytes venomen
dc.titleMTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage65
dc.citation.other192: 57-65
dc.citation.spage57
dc.citation.volume192
dc.identifier.doi10.1016/j.toxicon.2021.01.012
dc.identifier.rcubconv_586
dc.identifier.scopus2-s2.0-85099912728
dc.type.versionpublishedVersion


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