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dc.creatorPilipović, Ivan
dc.creatorStojić-Vukanić, Zorica
dc.creatorPrijić, Ivana
dc.creatorJasnić, Nebojša
dc.creatorLeposavić, Gordana
dc.date.accessioned2021-02-18T10:53:28Z
dc.date.available2021-02-18T10:53:28Z
dc.date.issued2020
dc.identifier.issn0969-9961
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/561
dc.description.abstractSympathetic dysfunction is suggested to contribute to development of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE) alike. Considering important role of microglia in development/resolution of neuroinflammation, contribution of noradrenaline, the key sympathetic end-point mediator, in modulation of microglial phenotypic and functional properties in rat EAE model was examined. The study showed that noradrenaline acting in neurocrine and autocrine/paracrine way might influence microglia during EAE. Propranolol treatment over the effector phase moderated EAE course. This was associated with the increased proportion of microglia expressing CX3CR1, the key molecule in their immunomodulatory/neuroprotective action, and up regulation of CX3CR1 downstream Nrf2 gene. This correlated with the increased heme oxygenase-1 (HO-1) expression and phagocytic capacity of microglia, and their phenotypic changes mirrored in increased proportion of CD163- and CD83-expressing cells. The enhanced HO-1 expression was linked with the decreased proportion of microglial cells expressing IL-1 beta and IL-23, and possibly IL-6, followed by increased proportion of IL-10 expressing microglia, and downregulated MCP-1/CCL2 expression. Consistently, spinal cord infiltration with blood-borne myeloid and CD4 + T cells, as well as CD4 + T-cell reactivation/proliferation and differentiation into highly pathogenic IL-17 + cells co-producing IFN-gamma and GM-CSF were decreased in propranolol-treated rats compared with saline-injected controls. The in vitro investigations of the effects of noradrenaline on microglia showed that noradrenaline through beta-adrenoceptor may influence Nrf2 expression also via CX3CR1-independent route. The study suggests beta-adrenoceptor-mediated neuroinflammation-promoting role of noradrenaline in EAE via modulation of microglial Nrf2 expression, and thereby forms the basis for further translational pharmacological research to improve multiple sclerosis therapy.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsopenAccess
dc.sourceNeurobiology of Disease
dc.subjectExperimental autoimmune encephalomyelitisen
dc.subjectNoradrenalineen
dc.subjectbeta-Adrenoceptoren
dc.subjectMicrogliaen
dc.subjectCX3CR1en
dc.subjectNrf2/HO-1 axisen
dc.titlePropranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microgliaen
dc.typearticle
dc.rights.licenseARR
dc.citation.other134
dc.citation.rankM21
dc.citation.volume134
dc.identifier.doi10.1016/j.nbd.2019.104665
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/372/558.pdf
dc.identifier.pmid31689515
dc.identifier.scopus2-s2.0-85074637402
dc.identifier.wos000509818400026
dc.type.versionpublishedVersion


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