Приказ основних података о документу

dc.creatorStojić-Vukanić, Zorica
dc.creatorPilipović, Ivan
dc.creatorBufan, Biljana
dc.creatorStojanović, Marija
dc.creatorLeposavić, Gordana
dc.date.accessioned2021-02-18T10:53:24Z
dc.date.available2021-02-18T10:53:24Z
dc.date.issued2020
dc.identifier.issn1389-5729
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/560
dc.description.abstractThe study investigated influence of sex and age on splenic myeloid dendritic cells (DCs) from Dark Agouti rats. Freshly isolated DCs from young males exhibited less mature phenotype and greater endocytic capacity compared with those from age-matched females. Upon LPS stimulation in vitro they were less potent in stimulating allogeneic CD4+ cells in mixed leukocyte reaction (MLR), due to lower expression of MHC II, and greater NO and IL-10 production. In accordance with higher TGF-beta production, young male rat DCs were less potent in stimulating IL-17 production in MLR than those from young females. Irrespective of sex, endocytic capacity and responsiveness of DCs to LPS stimulation in culture, judging by their allostimulatory capacity in MLR decreased with age, reflecting decline in MHC II surface density followed by their greater NO production; the effects more prominent in females. Additionally, compared with LPS-stimulated DCs from young rats, those from sex-matched aged rats were more potent in stimulating IL-10 production in MLR, whereas capacity of DCs from aged female and male rats to stimulate IL-17 production remained unaltered and decreased, respectively. This reflected age-related shift in IL-6/TGF-beta production level ratio in LPS-stimulated DC cultures towards TGF-beta, and sex-specific age-related remodeling CD4+ cell cytokine pathways. Additionally, compared with LPS-stimulated DCs from young rats, those cells from sex-matched aged rats were less potent in stimulating IFN-gamma production in MLR, the effect particularly prominent in MLRs encompassing male rat DCs. The study showed that stimulatory and polarizing capacity of DCs depends on rat sex and age.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsrestrictedAccess
dc.sourceBiogerontology
dc.subjectAgingen
dc.subjectSex differencesen
dc.subjectMyeloid dendritic cellsen
dc.subjectAllostimulatory capacityen
dc.subjectCytokine secretory profileen
dc.titleAge and sex determine CD4+T cell stimulatory and polarizing capacity of rat splenic dendritic cellsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage107
dc.citation.issue1
dc.citation.other21(1): 83-107
dc.citation.rankM21
dc.citation.spage83
dc.citation.volume21
dc.identifier.doi10.1007/s10522-019-09845-y
dc.identifier.pmid31646402
dc.identifier.scopus2-s2.0-85074022782
dc.identifier.wos000492172900002
dc.type.versionpublishedVersion


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Приказ основних података о документу