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dc.creatorRadosavljević, Jelena
dc.creatorApostolović, Danijela
dc.creatorMihailović, Jelena
dc.creatorAtanasković-Marković, Marina
dc.creatorBurazer, Lidija
dc.creatorvan Hage, Marianne
dc.creatorĆirković-Veličković, Tanja
dc.date.accessioned2021-02-18T10:52:29Z
dc.date.available2021-02-18T10:52:29Z
dc.date.issued2020
dc.identifier.issn2304-8158
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/547
dc.description.abstractThe aim of this study was to identify short digestion-resistant peptides (SDRPs) released by pepsin digestion of the whole cow's milk and examine their IgE reactivity and allergenicity. Raw milk was subjected to simulated gastric digestion. SDRPs were fractionated from the digests and identified by MS. Milk SDRPs were evaluated for aggregability, propensity to compete for IgE binding with individual milk allergens, and ability to bind IgG4 from allergic and milk-tolerant individuals. The majority of milk SDRPs originated from caseins (97% of peptides) and overlapped with the known IgE epitopes of cow's milk allergens. SDRPs competed with milk proteins for binding to human IgE and readily formed aggregates. The average peptide length was 10.6 +/- 3.5 amino acids. The ability to provoke allergenic in vivo responses was confirmed by skin-prick testing (SPT) in five milk-allergic subjects. This was attributed to the peptide ability to aggregate into non-covalent complexes. SDRPs are able to induce response in SPT, but only in 50% of the sera SDRPs were able to inhibit IgG4 binding to caseins. Hence, SDRPs corresponding to the mainly continuous epitopes of milk proteins induce allergenic in vivo responses in milk-allergic subjects due to aggregation.en
dc.publisherMDPI, Basel
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.relationBelgian Special Research Fund BOF StG [01N01718]
dc.relationSerbian Academy of Sciences and Arts GA [F-26]
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/810752/EU//
dc.relationSwedish Research CouncilSwedish Research Council
dc.relationStockholm County Council (ALF Project)Stockholm County Council
dc.relationSwedish Asthma and Allergy Association's Research Foundation
dc.relationSwedish Heart-Lung FoundationSwedish Heart-Lung Foundation
dc.relationSwedish Cancer and Allergy Foundation
dc.relationKonsul Th C Berg's Foundation
dc.relationKing Gustaf V's 80-year Foundation
dc.relationMagnus Bergvall Foundation
dc.relationLars Hierta Memorial Foundation
dc.relationKarolinska InstitutetKarolinska Institutet
dc.rightsopenAccess
dc.sourceFoods
dc.subjectmilken
dc.subjectallergenicityen
dc.subjectallergyen
dc.subjectpepsinen
dc.subjectcaseinen
dc.subjectIgEen
dc.subjectdigestion-resistant peptidesen
dc.subjectgastric simulated digestionen
dc.titleDigestomics of Cow's Milk: Short Digestion-Resistant Peptides of Casein Form Functional Complexes by Aggregationen
dc.typearticle
dc.rights.licenseARR
dc.citation.issue11
dc.citation.other9(11)
dc.citation.rankM21
dc.citation.volume9
dc.identifier.doi10.3390/foods9111576
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/363/544.pdf
dc.identifier.pmid33143174
dc.identifier.rcubconv_485
dc.identifier.wos000593376300001
dc.type.versionpublishedVersion


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