Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction
Samo za registrovane korisnike
2020
Autori
Micić, MilutinAntonijević, Đorđe
Milutinović-Smiljanić, Sanja
Trisić, Dijana
Colović, Božana
Kosanović, Dejana
Prokić, Bogomir
Vasić, Jugoslav
Zivković, Slavoljub
Milašin, Jelena
Danilović, Vesna
Đurić, Marija
Jokanović, Vukoman
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 mu m. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presen...ce of bone formation indicators - bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
Ključne reči:
3D printing / bone reconstruction / personalized medicine / tissue engineeringIzvor:
Biomedical Engineering-Biomedizinische Technik, 2020, 65, 4, 491-505Izdavač:
- Walter De Gruyter Gmbh, Berlin
Finansiranje / projekti:
- Hemijsko i strukturno dizajniranje nanomaterijala za primenu u medicini i inženjerstvu tkiva (RS-172026)
- Funkcionalni, funkcionalizovani i usavršeni nano materijali (RS-45005)
Napomena:
- Link to erratum: https://intor.torlakinstitut.com/handle/123456789/660
- Supplementary information: https://hdl.handle.net/21.15107/rcub_intor_661
Povezane informacije:
- Povezani sadržaj
https://intor.torlakinstitut.com/handle/123456789/660 - Povezani sadržaj
https://hdl.handle.net/21.15107/rcub_intor_661
DOI: 10.1515/bmt-2019-0218
ISSN: 0013-5585
PubMed: 32335536
WoS: 000560600600012
Scopus: 2-s2.0-85084702256
Institucija/grupa
TorlakTY - JOUR AU - Micić, Milutin AU - Antonijević, Đorđe AU - Milutinović-Smiljanić, Sanja AU - Trisić, Dijana AU - Colović, Božana AU - Kosanović, Dejana AU - Prokić, Bogomir AU - Vasić, Jugoslav AU - Zivković, Slavoljub AU - Milašin, Jelena AU - Danilović, Vesna AU - Đurić, Marija AU - Jokanović, Vukoman PY - 2020 UR - http://intor.torlakinstitut.com/handle/123456789/542 AB - The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 mu m. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators - bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale. PB - Walter De Gruyter Gmbh, Berlin T2 - Biomedical Engineering-Biomedizinische Technik T1 - Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction EP - 505 IS - 4 SP - 491 VL - 65 DO - 10.1515/bmt-2019-0218 ER -
@article{ author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trisić, Dijana and Colović, Božana and Kosanović, Dejana and Prokić, Bogomir and Vasić, Jugoslav and Zivković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija and Jokanović, Vukoman", year = "2020", abstract = "The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 mu m. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators - bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.", publisher = "Walter De Gruyter Gmbh, Berlin", journal = "Biomedical Engineering-Biomedizinische Technik", title = "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction", pages = "505-491", number = "4", volume = "65", doi = "10.1515/bmt-2019-0218" }
Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trisić, D., Colović, B., Kosanović, D., Prokić, B., Vasić, J., Zivković, S., Milašin, J., Danilović, V., Đurić, M.,& Jokanović, V.. (2020). Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedical Engineering-Biomedizinische Technik Walter De Gruyter Gmbh, Berlin., 65(4), 491-505. https://doi.org/10.1515/bmt-2019-0218
Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trisić D, Colović B, Kosanović D, Prokić B, Vasić J, Zivković S, Milašin J, Danilović V, Đurić M, Jokanović V. Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedical Engineering-Biomedizinische Technik. 2020;65(4):491-505. doi:10.1515/bmt-2019-0218 .
Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trisić, Dijana, Colović, Božana, Kosanović, Dejana, Prokić, Bogomir, Vasić, Jugoslav, Zivković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija, Jokanović, Vukoman, "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction" in Biomedical Engineering-Biomedizinische Technik, 65, no. 4 (2020):491-505, https://doi.org/10.1515/bmt-2019-0218 . .