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Propranolol Impairs Primary Immune Responses in Rat Experimental Autoimmune Encephalomyelitis

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Authors
Pilipović, Ivan
Vujnović, Ivana
Petrović, Raisa
Stojić-Vukanić, Zorica
Leposavić, Gordana
Article (Published version)
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Abstract
Objective: We examined the effect of beta-adrenoceptor (AR) blockade in the preclinical phase of experimental autoimmune encephalomyelitis (EAE), the most commonly used model of multiple sclerosis, on the development of primary CD4+ T-cell responses in draining lymph nodes (dLNs). Methods: CD11b+ cell migration to dLNs, CD4+ T-cell activation/proliferation, and IL-17+ CD4+ (Th17) cell numbers in dLN and spinal cord (SC) were examined in male and female Dark Agouti rats using flow cytometry analysis. Results: Irrespective of sex, in propranolol-treated (PT) rats, migration of CD11b+ antigen-presenting cells from the site of immunization to dLNs was impaired compared with saline-treated controls and consequently the frequency of all CD11b+ cells in dLNs and activated cells among them, too. This correlated with decreased expression of CCL19/21 transcripts in dLNs. Consistently, the frequency of activated/proliferating cells among dLN CD4+ T cells was reduced in PT rats. Additionally, prop...ranolol reduced the number of Th17 cells in dLNs and SC. Consistently, male and female PT rats exhibited a decreased incidence of EAE and prolonged duration of the asymptomatic disease phase. Conclusion: This study suggests that sympathetic dysregulation is involved in the outbreak of clinical EAE. (C) 2019 S. Karger AG, Basel

Keywords:
Experimental autoimmune encephalomyelitis / Th17 lymphocytes / Antigen-presenting cell migration / beta-Adrenoceptor / CCL19 / CCL21
Source:
Neuroimmunomodulation, 2019, 26, 3, 129-138
Publisher:
  • Karger, Basel
Funding / projects:
  • Immune system plasticity during aging: Immunomodulatory capacity of oestrogens (RS-175050)

DOI: 10.1159/000500094

ISSN: 1021-7401

PubMed: 31132768

WoS: 000481456200003

Scopus: 2-s2.0-85066936523
[ Google Scholar ]
4
3
URI
http://intor.torlakinstitut.com/handle/123456789/541
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Pilipović, Ivan
AU  - Vujnović, Ivana
AU  - Petrović, Raisa
AU  - Stojić-Vukanić, Zorica
AU  - Leposavić, Gordana
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/541
AB  - Objective: We examined the effect of beta-adrenoceptor (AR) blockade in the preclinical phase of experimental autoimmune encephalomyelitis (EAE), the most commonly used model of multiple sclerosis, on the development of primary CD4+ T-cell responses in draining lymph nodes (dLNs). Methods: CD11b+ cell migration to dLNs, CD4+ T-cell activation/proliferation, and IL-17+ CD4+ (Th17) cell numbers in dLN and spinal cord (SC) were examined in male and female Dark Agouti rats using flow cytometry analysis. Results: Irrespective of sex, in propranolol-treated (PT) rats, migration of CD11b+ antigen-presenting cells from the site of immunization to dLNs was impaired compared with saline-treated controls and consequently the frequency of all CD11b+ cells in dLNs and activated cells among them, too. This correlated with decreased expression of CCL19/21 transcripts in dLNs. Consistently, the frequency of activated/proliferating cells among dLN CD4+ T cells was reduced in PT rats. Additionally, propranolol reduced the number of Th17 cells in dLNs and SC. Consistently, male and female PT rats exhibited a decreased incidence of EAE and prolonged duration of the asymptomatic disease phase. Conclusion: This study suggests that sympathetic dysregulation is involved in the outbreak of clinical EAE. (C) 2019 S. Karger AG, Basel
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - Propranolol Impairs Primary Immune Responses in Rat Experimental Autoimmune Encephalomyelitis
EP  - 138
IS  - 3
SP  - 129
VL  - 26
DO  - 10.1159/000500094
UR  - conv_458
ER  - 
@article{
author = "Pilipović, Ivan and Vujnović, Ivana and Petrović, Raisa and Stojić-Vukanić, Zorica and Leposavić, Gordana",
year = "2019",
abstract = "Objective: We examined the effect of beta-adrenoceptor (AR) blockade in the preclinical phase of experimental autoimmune encephalomyelitis (EAE), the most commonly used model of multiple sclerosis, on the development of primary CD4+ T-cell responses in draining lymph nodes (dLNs). Methods: CD11b+ cell migration to dLNs, CD4+ T-cell activation/proliferation, and IL-17+ CD4+ (Th17) cell numbers in dLN and spinal cord (SC) were examined in male and female Dark Agouti rats using flow cytometry analysis. Results: Irrespective of sex, in propranolol-treated (PT) rats, migration of CD11b+ antigen-presenting cells from the site of immunization to dLNs was impaired compared with saline-treated controls and consequently the frequency of all CD11b+ cells in dLNs and activated cells among them, too. This correlated with decreased expression of CCL19/21 transcripts in dLNs. Consistently, the frequency of activated/proliferating cells among dLN CD4+ T cells was reduced in PT rats. Additionally, propranolol reduced the number of Th17 cells in dLNs and SC. Consistently, male and female PT rats exhibited a decreased incidence of EAE and prolonged duration of the asymptomatic disease phase. Conclusion: This study suggests that sympathetic dysregulation is involved in the outbreak of clinical EAE. (C) 2019 S. Karger AG, Basel",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "Propranolol Impairs Primary Immune Responses in Rat Experimental Autoimmune Encephalomyelitis",
pages = "138-129",
number = "3",
volume = "26",
doi = "10.1159/000500094",
url = "conv_458"
}
Pilipović, I., Vujnović, I., Petrović, R., Stojić-Vukanić, Z.,& Leposavić, G.. (2019). Propranolol Impairs Primary Immune Responses in Rat Experimental Autoimmune Encephalomyelitis. in Neuroimmunomodulation
Karger, Basel., 26(3), 129-138.
https://doi.org/10.1159/000500094
conv_458
Pilipović I, Vujnović I, Petrović R, Stojić-Vukanić Z, Leposavić G. Propranolol Impairs Primary Immune Responses in Rat Experimental Autoimmune Encephalomyelitis. in Neuroimmunomodulation. 2019;26(3):129-138.
doi:10.1159/000500094
conv_458 .
Pilipović, Ivan, Vujnović, Ivana, Petrović, Raisa, Stojić-Vukanić, Zorica, Leposavić, Gordana, "Propranolol Impairs Primary Immune Responses in Rat Experimental Autoimmune Encephalomyelitis" in Neuroimmunomodulation, 26, no. 3 (2019):129-138,
https://doi.org/10.1159/000500094 .,
conv_458 .

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