Приказ основних података о документу

dc.creatorStanojević, Maja
dc.creatorĆirković, Valentina
dc.creatorSiljić, Marina
dc.creatorGligić, Ana
dc.creatorStamenković, Gorana
dc.date.accessioned2021-02-18T10:51:17Z
dc.date.available2021-02-18T10:51:17Z
dc.date.issued2019
dc.identifier.issn1876-0341
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/530
dc.description.abstractBackground: Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. Methods: Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral anti-bodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. Results: PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. Conclusions: Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation. (C) 2019 Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en
dc.publisherElsevier Science London, London
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175024/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of Infection and Public Health
dc.subjectDobrava-Belgrade hantavirusen
dc.subjectSerbiaen
dc.subjectMolecularen
dc.titleMolecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011en
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage649
dc.citation.issue5
dc.citation.other12(5): 645-649
dc.citation.rankM22
dc.citation.spage645
dc.citation.volume12
dc.identifier.doi10.1016/j.jiph.2019.02.021
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/348/527.pdf
dc.identifier.pmid30910417
dc.identifier.scopus2-s2.0-85063220967
dc.identifier.wos000484679000010
dc.type.versionpublishedVersion


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Приказ основних података о документу