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dc.creatorBlagojević, Veljko
dc.creatorKovačević-Jovanović, Vesna
dc.creatorĆuruvija, Ivana
dc.creatorPetrović, Raisa
dc.creatorVujnović, Ivana
dc.creatorVujić, Vesna
dc.creatorStanojević, Stanislava
dc.date.accessioned2021-02-18T10:50:05Z
dc.date.available2021-02-18T10:50:05Z
dc.date.issued2018
dc.identifier.issn0024-3205
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/512
dc.description.abstractAims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsrestrictedAccess
dc.sourceLife Sciences
dc.subjectAlbino Oxford (AO) rat strainen
dc.subjectDark Agouti (DA) rat strainen
dc.subjectE. colien
dc.subjectEnterococcus spp.en
dc.subjectperitoneal immune cellsen
dc.titleRat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?en
dc.typearticle
dc.rights.licenseARR
dc.citation.epage157
dc.citation.other197: 147-157
dc.citation.rankM21
dc.citation.spage147
dc.citation.volume197
dc.identifier.doi10.1016/j.lfs.2018.02.011
dc.identifier.pmid29427649
dc.identifier.rcubconv_426
dc.identifier.scopus2-s2.0-85042137776
dc.identifier.wos000426435700018
dc.type.versionpublishedVersion


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