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dc.creatorInić-Kanada, Aleksandra
dc.creatorStein, Elisabeth
dc.creatorStojanović, Marijana
dc.creatorSchuerer, Nadine
dc.creatorGhasemian, Ehsan
dc.creatorFilipović, Ana
dc.creatorMarinković, Emilija
dc.creatorKosanović, Dejana
dc.creatorBarisani-Asenbauer, Talin
dc.date.accessioned2021-02-18T10:49:24Z
dc.date.available2021-02-18T10:49:24Z
dc.date.issued2018
dc.identifier.issn0921-8971
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/502
dc.description.abstractOcular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.en
dc.publisherSpringer, Dordrecht
dc.relationLaura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsopenAccess
dc.sourceJournal of Applied Phycology
dc.subjectCarrageenanen
dc.subjectTrachomaen
dc.subjectNatural productsen
dc.subjectChlamydiaen
dc.titleEffects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivoen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage2610
dc.citation.issue4
dc.citation.other30(4): 2601-2610
dc.citation.rankM21
dc.citation.spage2601
dc.citation.volume30
dc.identifier.doi10.1007/s10811-018-1435-0
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/323/499.pdf
dc.identifier.pmid30147240
dc.identifier.rcubconv_438
dc.identifier.scopus2-s2.0-85043697809
dc.identifier.wos000440782100039
dc.type.versionpublishedVersion


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