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dc.creatorPetrović, Raisa
dc.creatorBufan, Biljana
dc.creatorArsenović-Ranin, Nevena
dc.creatorŽivković, Irena
dc.creatorMinić, Rajna
dc.creatorRadojević, Katarina
dc.creatorLeposavić, Gordana
dc.date.accessioned2021-02-18T10:49:20Z
dc.date.available2021-02-18T10:49:20Z
dc.date.issued2018
dc.identifier.issn0024-3205
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/501
dc.description.abstractAims: The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV). Main methods: C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-gamma and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively. Key findings: BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sexmatched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antigen-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-gamma than those from C57BL/6 mice. Significance: The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceLife Sciences
dc.subjectMouse genetic backgrounden
dc.subjectInfluenza vaccineen
dc.subjectAntibody responseen
dc.subjectGerminal center reactionen
dc.subjectSex differencesen
dc.titleMouse strain and sex as determinants of immune response to trivalent influenza vaccineen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage126
dc.citation.other207: 117-126
dc.citation.rankM21
dc.citation.spage117
dc.citation.volume207
dc.identifier.doi10.1016/j.lfs.2018.05.056
dc.identifier.pmid29859986
dc.identifier.rcubconv_437
dc.identifier.scopus2-s2.0-85048462717
dc.identifier.wos000439876800015
dc.type.versionpublishedVersion


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