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dc.creatorFilipović, Ana
dc.creatorGhasemian, Ehsan
dc.creatorInić-Kanada, Aleksandra
dc.creatorLukić, Ivana
dc.creatorStein, Elisabeth
dc.creatorMarinković, Emilija
dc.creatorĐokić, Radmila
dc.creatorKosanović, Dejana
dc.creatorSchuerer, Nadine
dc.creatorChalabi, Hadeel
dc.creatorBelij-Rammerstorfer, Sandra
dc.creatorStojanović, Marijana
dc.creatorBarisani-Asenbauer, Talin
dc.date.accessioned2021-02-18T10:47:43Z
dc.date.available2021-02-18T10:47:43Z
dc.date.issued2017
dc.identifier.issn1932-6203
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/477
dc.description.abstractFollowing infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.en
dc.publisherPublic Library Science, San Francisco
dc.relationLaura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationOeAD - die osterreichische Agentur fur internationale Mobilitat und Kooperation in Bildung, Wissensc
dc.relation451-03-01039/2015-09/04
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS One
dc.titleThe effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infectionen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue7
dc.citation.other12(7)
dc.citation.rankM21
dc.citation.volume12
dc.identifier.doi10.1371/journal.pone.0180551
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/301/474.pdf
dc.identifier.pmid28678871
dc.identifier.rcubconv_416
dc.identifier.scopus2-s2.0-85022080375
dc.identifier.wos000405272200073
dc.type.versionpublishedVersion


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