Show simple item record

dc.creatorMitić, Katarina
dc.creatorMuhandes, Lina
dc.creatorMinić, Rajna
dc.creatorPetrušić, Vladimir
dc.creatorŽivković, Irena
dc.date.accessioned2021-02-18T10:46:50Z
dc.date.available2021-02-18T10:46:50Z
dc.date.issued2016
dc.identifier.issn0015-5500
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/464
dc.description.abstractTesting of every new vaccine involves investigation of its immunogenicity, which is based on monitoring its ability to induce specific antibodies in animals. The fastest and most sensitive method used for this purpose is enzyme-linked immunosorbent assay (ELISA). However, commercial ELISA kits with whole influenza virus antigens are not available on the market, and it is therefore essential to establish an adequate assay for testing influenza virus-specific antibodies. We developed ELISA with whole influenza virus strains for the season 2011/2012 as antigens and validated it by checking its specificity, accuracy, linearity, range, precision, and sensitivity. The results show that we developed high-quality ELISA that can be used to test immunogenicity of newly produced seasonal or pandemic vaccines in mice. The pre-existence of validated ELISA enables shortening the time from the process of vaccine production to its use in patients, which is particularly important in the case of a pandemic.en
dc.publisherCharles Univ Prague, First Faculty Medicine, Prague 6
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceFolia Biologica (Czech Republic)
dc.subjectELISAen
dc.subjectinfluenzaen
dc.subjectvalidationen
dc.subjectpandemicen
dc.titleOptimization and Validation of ELISA for Pre-Clinical Trials of Influenza Vaccineen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage249
dc.citation.issue6
dc.citation.other62(6): 241-249
dc.citation.rankM23
dc.citation.spage241
dc.citation.volume62
dc.identifier.pmid28189147
dc.identifier.rcubconv_409
dc.identifier.scopus2-s2.0-85013290685
dc.identifier.wos000398215600003
dc.type.versionpublishedVersion


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record