Show simple item record

dc.creatorInić-Kanada, Aleksandra
dc.creatorStojanović, Marijana
dc.creatorMarinković, Emilija
dc.creatorBecker, Elisabeth
dc.creatorStein, Elisabeth
dc.creatorLukić, Ivana
dc.creatorĐokić, Radmila
dc.creatorSchuerer, Nadine
dc.creatorHegemann, Johannes H.
dc.creatorBarisani-Asenbauer, Talin
dc.date.accessioned2021-02-18T10:46:14Z
dc.date.available2021-02-18T10:46:14Z
dc.date.issued2016
dc.identifier.issn1932-6203
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/455
dc.description.abstractRecent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development.en
dc.publisherPublic Library Science, San Francisco
dc.relationLaura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS One
dc.titleA Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein Cen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue9
dc.citation.other11(9)
dc.citation.rankM21
dc.citation.volume11
dc.identifier.doi10.1371/journal.pone.0157875
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/285/452.pdf
dc.identifier.pmid27636704
dc.identifier.rcubconv_393
dc.identifier.scopus2-s2.0-84992427898
dc.identifier.wos000383723700001
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record