Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats
Authors
Kovačević-Jovanović, VesnaMiletić, Tatjana
Stanojević, Stanislava
Mitić, Katarina
Dimitrijević, Mirjana
Article (Published version)
Metadata
Show full item recordAbstract
We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serolo...gic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.
Keywords:
adjuvant arthritis / commensal bacteria / Escherichia coli / humoral immune response / rat strainsSource:
Acta Microbiologica et Immunologica Hungarica, 2015, 62, 1, 1-19Publisher:
- Akademiai Kiado Zrt, Budapest
Funding / projects:
DOI: 10.1556/AMicr.62.2015.1.1
ISSN: 1217-8950
PubMed: 25823450
WoS: 000352094200001
Scopus: 2-s2.0-84926289399
Collections
Institution/Community
TorlakTY - JOUR AU - Kovačević-Jovanović, Vesna AU - Miletić, Tatjana AU - Stanojević, Stanislava AU - Mitić, Katarina AU - Dimitrijević, Mirjana PY - 2015 UR - http://intor.torlakinstitut.com/handle/123456789/449 AB - We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Microbiologica et Immunologica Hungarica T1 - Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats EP - 19 IS - 1 SP - 1 VL - 62 DO - 10.1556/AMicr.62.2015.1.1 ER -
@article{ author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana", year = "2015", abstract = "We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Microbiologica et Immunologica Hungarica", title = "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats", pages = "19-1", number = "1", volume = "62", doi = "10.1556/AMicr.62.2015.1.1" }
Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2015). Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica Akademiai Kiado Zrt, Budapest., 62(1), 1-19. https://doi.org/10.1556/AMicr.62.2015.1.1
Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica. 2015;62(1):1-19. doi:10.1556/AMicr.62.2015.1.1 .
Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats" in Acta Microbiologica et Immunologica Hungarica, 62, no. 1 (2015):1-19, https://doi.org/10.1556/AMicr.62.2015.1.1 . .