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dc.creatorMinić, Rajna
dc.creatorGavrović-Jankulović, Marija
dc.creatorPetrušić, Vladimir
dc.creatorŽivković, Irena
dc.creatorEijsink, Vincent G.H.
dc.creatorDimitrijević, Ljiljana
dc.creatorMathiesen, Geir
dc.date.accessioned2021-02-18T10:45:41Z
dc.date.available2021-02-18T10:45:41Z
dc.date.issued2015
dc.identifier.issn0168-1656
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/447
dc.description.abstractGroup I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinant Fes p1 allergen, from Festuca pratensis was tested in a mouse model of Fes p1 allergy. For surface expression of Fes p1 allergen in L. plantarum WCFS1 pSIP system with inducible expression was used. Balb/c mice were sensitized with Fes p1 protein in alum and subsequently received live recombinant L. plantarum orally. Antibody levels (IgE, total IgG, IgG1, IgG2a, and IgA) were determined by ELISA. Differential eosinophil count in peripheral blood was performed. Reduced peripheral blood eosinophilia and increased serum IgG2A levels was detected in both groups which received live L. plantarum orally. Specific serum IgA levels were increased only in mice treated with the recombinant bacteria. Oral application of L. plantarum WCFS1 has a beneficial therapeutic effect in a mouse model of Fes p1 allergy. Cell surface expression of Fes p1 allergen potentiates this phenomenon in an allergen specific way. (C) 2015 Elsevier B.V. All rights reserved.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Biotechnology
dc.subjectAllergyen
dc.subjectOral therapyen
dc.subjectRecombinant L. plantarum WCFS1en
dc.subjectFestuca pratensisen
dc.titleEffects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in miceen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage28
dc.citation.other199: 23-28
dc.citation.rankM22
dc.citation.spage23
dc.citation.volume199
dc.identifier.doi10.1016/j.jbiotec.2015.01.028
dc.identifier.pmid25687100
dc.identifier.rcubconv_357
dc.identifier.scopus2-s2.0-84923261413
dc.identifier.wos000351660600005
dc.type.versionpublishedVersion


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