Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution o...f DOBV in its reservoir hosts in Serbia.
TY - JOUR
AU - Stanojević, Maja
AU - Nikolić, V.
AU - Stajković, Novica
AU - Stamenković, Gorana
AU - Božović, Bojana
AU - Čekanac, Radovan
AU - Marušić, Predrag
AU - Gligić, Ana
PY - 2015
UR - http://intor.torlakinstitut.com/handle/123456789/439
AB - Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia.
PB - Cambridge Univ Press, New York
T2 - Epidemiology and Infection
T1 - Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia
EP - 404
IS - 2
SP - 400
VL - 143
DO - 10.1017/S0950268814001010
UR - conv_352
ER -
@article{
author = "Stanojević, Maja and Nikolić, V. and Stajković, Novica and Stamenković, Gorana and Božović, Bojana and Čekanac, Radovan and Marušić, Predrag and Gligić, Ana",
year = "2015",
abstract = "Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia.",
publisher = "Cambridge Univ Press, New York",
journal = "Epidemiology and Infection",
title = "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia",
pages = "404-400",
number = "2",
volume = "143",
doi = "10.1017/S0950268814001010",
url = "conv_352"
}
Stanojević, M., Nikolić, V., Stajković, N., Stamenković, G., Božović, B., Čekanac, R., Marušić, P.,& Gligić, A.. (2015). Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia. in Epidemiology and Infection
Cambridge Univ Press, New York., 143(2), 400-404.
https://doi.org/10.1017/S0950268814001010
conv_352
Stanojević M, Nikolić V, Stajković N, Stamenković G, Božović B, Čekanac R, Marušić P, Gligić A. Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia. in Epidemiology and Infection. 2015;143(2):400-404.
doi:10.1017/S0950268814001010
conv_352 .
Stanojević, Maja, Nikolić, V., Stajković, Novica, Stamenković, Gorana, Božović, Bojana, Čekanac, Radovan, Marušić, Predrag, Gligić, Ana, "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia" in Epidemiology and Infection, 143, no. 2 (2015):400-404,
https://doi.org/10.1017/S0950268814001010 .,
conv_352 .
