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dc.creatorInić-Kanada, Aleksandra
dc.creatorStojanović, Marijana
dc.creatorSchlacher, Simone
dc.creatorStein, Elisabeth
dc.creatorBelij-Rammerstorfer, Sandra
dc.creatorMarinković, Emilija
dc.creatorLukić, Ivana
dc.creatorMontanaro, Jacqueline
dc.creatorSchuerer, Nadine
dc.creatorBintner, Nora
dc.creatorKovačević-Jovanović, Vesna
dc.creatorKrnjaja, Ognjen
dc.creatorMayr, Ulrike Beate
dc.creatorLubitz, Werner
dc.creatorBarisani-Asenbauer, Talin
dc.date.accessioned2021-02-18T10:44:47Z
dc.date.available2021-02-18T10:44:47Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/434
dc.description.abstractTrachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.en
dc.publisherPublic Library Science, San Francisco
dc.relationLaura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationBIRD-C
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS One
dc.titleDelivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriersen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue12
dc.citation.other10(12)
dc.citation.rankM21
dc.citation.volume10
dc.identifier.doi10.1371/journal.pone.0144380
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/266/431.pdf
dc.identifier.pmid26656797
dc.identifier.rcubconv_375
dc.identifier.scopus2-s2.0-84956569782
dc.identifier.wos000366903600041
dc.type.versionpublishedVersion


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