Evidence of recombination in Tula virus strains from Serbia
Samo za registrovane korisnike
2014
Autori
Nikolić, ValentinaStajković, Novica
Stamenković, Gorana
Čekanac, Radovan
Marušić, Predrag
Siljić, Marina
Gligić, Ana
Stanojević, Maja
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV... S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.
Ključne reči:
Recombination / Tula virus / Phylogenetic analysis / Molecular evolutionIzvor:
Infection Genetics and Evolution, 2014, 21, 472-478Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Filogenetski pristup analizi molekularne evolucije visoko varijabilnih virusa - koinfekcije, interakcija virusa i domaćina (RS-MESTD-Basic Research (BR or ON)-175024)
DOI: 10.1016/j.meegid.2013.08.020
ISSN: 1567-1348
PubMed: 24008094
WoS: 000332273800056
Scopus: 2-s2.0-84893774623
Institucija/grupa
TorlakTY - JOUR AU - Nikolić, Valentina AU - Stajković, Novica AU - Stamenković, Gorana AU - Čekanac, Radovan AU - Marušić, Predrag AU - Siljić, Marina AU - Gligić, Ana AU - Stanojević, Maja PY - 2014 UR - http://intor.torlakinstitut.com/handle/123456789/414 AB - Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved. PB - Elsevier, Amsterdam T2 - Infection Genetics and Evolution T1 - Evidence of recombination in Tula virus strains from Serbia EP - 478 SP - 472 VL - 21 DO - 10.1016/j.meegid.2013.08.020 ER -
@article{ author = "Nikolić, Valentina and Stajković, Novica and Stamenković, Gorana and Čekanac, Radovan and Marušić, Predrag and Siljić, Marina and Gligić, Ana and Stanojević, Maja", year = "2014", abstract = "Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "Infection Genetics and Evolution", title = "Evidence of recombination in Tula virus strains from Serbia", pages = "478-472", volume = "21", doi = "10.1016/j.meegid.2013.08.020" }
Nikolić, V., Stajković, N., Stamenković, G., Čekanac, R., Marušić, P., Siljić, M., Gligić, A.,& Stanojević, M.. (2014). Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution Elsevier, Amsterdam., 21, 472-478. https://doi.org/10.1016/j.meegid.2013.08.020
Nikolić V, Stajković N, Stamenković G, Čekanac R, Marušić P, Siljić M, Gligić A, Stanojević M. Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution. 2014;21:472-478. doi:10.1016/j.meegid.2013.08.020 .
Nikolić, Valentina, Stajković, Novica, Stamenković, Gorana, Čekanac, Radovan, Marušić, Predrag, Siljić, Marina, Gligić, Ana, Stanojević, Maja, "Evidence of recombination in Tula virus strains from Serbia" in Infection Genetics and Evolution, 21 (2014):472-478, https://doi.org/10.1016/j.meegid.2013.08.020 . .