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Evidence of recombination in Tula virus strains from Serbia

Authorized Users Only
2014
Authors
Nikolić, Valentina
Stajković, Novica
Stamenković, Gorana
Čekanac, Radovan
Marušić, Predrag
Siljić, Marina
Gligić, Ana
Stanojević, Maja
Article (Published version)
Metadata
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Abstract
Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV... S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.

Keywords:
Recombination / Tula virus / Phylogenetic analysis / Molecular evolution
Source:
Infection Genetics and Evolution, 2014, 21, 472-478
Publisher:
  • Elsevier, Amsterdam
Funding / projects:
  • Phylogenetic anaysis and molecular evolution of highly variable viruses: coinfections, host-pathogene interactions (RS-175024)

DOI: 10.1016/j.meegid.2013.08.020

ISSN: 1567-1348

PubMed: 24008094

WoS: 000332273800056

Scopus: 2-s2.0-84893774623
[ Google Scholar ]
16
16
URI
http://intor.torlakinstitut.com/handle/123456789/414
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Nikolić, Valentina
AU  - Stajković, Novica
AU  - Stamenković, Gorana
AU  - Čekanac, Radovan
AU  - Marušić, Predrag
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stanojević, Maja
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/414
AB  - Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Infection Genetics and Evolution
T1  - Evidence of recombination in Tula virus strains from Serbia
EP  - 478
SP  - 472
VL  - 21
DO  - 10.1016/j.meegid.2013.08.020
UR  - conv_334
ER  - 
@article{
author = "Nikolić, Valentina and Stajković, Novica and Stamenković, Gorana and Čekanac, Radovan and Marušić, Predrag and Siljić, Marina and Gligić, Ana and Stanojević, Maja",
year = "2014",
abstract = "Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Infection Genetics and Evolution",
title = "Evidence of recombination in Tula virus strains from Serbia",
pages = "478-472",
volume = "21",
doi = "10.1016/j.meegid.2013.08.020",
url = "conv_334"
}
Nikolić, V., Stajković, N., Stamenković, G., Čekanac, R., Marušić, P., Siljić, M., Gligić, A.,& Stanojević, M.. (2014). Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution
Elsevier, Amsterdam., 21, 472-478.
https://doi.org/10.1016/j.meegid.2013.08.020
conv_334
Nikolić V, Stajković N, Stamenković G, Čekanac R, Marušić P, Siljić M, Gligić A, Stanojević M. Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution. 2014;21:472-478.
doi:10.1016/j.meegid.2013.08.020
conv_334 .
Nikolić, Valentina, Stajković, Novica, Stamenković, Gorana, Čekanac, Radovan, Marušić, Predrag, Siljić, Marina, Gligić, Ana, Stanojević, Maja, "Evidence of recombination in Tula virus strains from Serbia" in Infection Genetics and Evolution, 21 (2014):472-478,
https://doi.org/10.1016/j.meegid.2013.08.020 .,
conv_334 .

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