Evidence of recombination in Tula virus strains from Serbia
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2014
Authors
Nikolić, Valentina
Stajković, Novica
Stamenković, Gorana

Čekanac, Radovan
Marušić, Predrag
Siljić, Marina

Gligić, Ana
Stanojević, Maja

Article (Published version)

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Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV... S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.
Keywords:
Recombination / Tula virus / Phylogenetic analysis / Molecular evolutionSource:
Infection Genetics and Evolution, 2014, 21, 472-478Publisher:
- Elsevier, Amsterdam
Funding / projects:
DOI: 10.1016/j.meegid.2013.08.020
ISSN: 1567-1348
PubMed: 24008094
WoS: 000332273800056
Scopus: 2-s2.0-84893774623
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TorlakTY - JOUR AU - Nikolić, Valentina AU - Stajković, Novica AU - Stamenković, Gorana AU - Čekanac, Radovan AU - Marušić, Predrag AU - Siljić, Marina AU - Gligić, Ana AU - Stanojević, Maja PY - 2014 UR - http://intor.torlakinstitut.com/handle/123456789/414 AB - Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved. PB - Elsevier, Amsterdam T2 - Infection Genetics and Evolution T1 - Evidence of recombination in Tula virus strains from Serbia EP - 478 SP - 472 VL - 21 DO - 10.1016/j.meegid.2013.08.020 UR - conv_334 ER -
@article{ author = "Nikolić, Valentina and Stajković, Novica and Stamenković, Gorana and Čekanac, Radovan and Marušić, Predrag and Siljić, Marina and Gligić, Ana and Stanojević, Maja", year = "2014", abstract = "Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "Infection Genetics and Evolution", title = "Evidence of recombination in Tula virus strains from Serbia", pages = "478-472", volume = "21", doi = "10.1016/j.meegid.2013.08.020", url = "conv_334" }
Nikolić, V., Stajković, N., Stamenković, G., Čekanac, R., Marušić, P., Siljić, M., Gligić, A.,& Stanojević, M.. (2014). Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution Elsevier, Amsterdam., 21, 472-478. https://doi.org/10.1016/j.meegid.2013.08.020 conv_334
Nikolić V, Stajković N, Stamenković G, Čekanac R, Marušić P, Siljić M, Gligić A, Stanojević M. Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution. 2014;21:472-478. doi:10.1016/j.meegid.2013.08.020 conv_334 .
Nikolić, Valentina, Stajković, Novica, Stamenković, Gorana, Čekanac, Radovan, Marušić, Predrag, Siljić, Marina, Gligić, Ana, Stanojević, Maja, "Evidence of recombination in Tula virus strains from Serbia" in Infection Genetics and Evolution, 21 (2014):472-478, https://doi.org/10.1016/j.meegid.2013.08.020 ., conv_334 .