Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains
Samo za registrovane korisnike
2014
Autori
Dimitrijević, MirjanaStanojević, Stanislava
Vujić, Vesna
Aleksić, Iva
Pilipović, Ivan
Leposavić, Gordana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 level...s decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.
Ključne reči:
Aging / Macrophages / Cytokines / NO/urea balance / DP4 / Rat strainsIzvor:
Biogerontology, 2014, 15, 5, 475-486Izdavač:
- Springer, New York
Finansiranje / projekti:
DOI: 10.1007/s10522-014-9513-4
ISSN: 1389-5729
PubMed: 25009084
WoS: 000342080300006
Scopus: 2-s2.0-84906936202
Institucija/grupa
TorlakTY - JOUR AU - Dimitrijević, Mirjana AU - Stanojević, Stanislava AU - Vujić, Vesna AU - Aleksić, Iva AU - Pilipović, Ivan AU - Leposavić, Gordana PY - 2014 UR - http://intor.torlakinstitut.com/handle/123456789/402 AB - Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats. PB - Springer, New York T2 - Biogerontology T1 - Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains EP - 486 IS - 5 SP - 475 VL - 15 DO - 10.1007/s10522-014-9513-4 ER -
@article{ author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Vujić, Vesna and Aleksić, Iva and Pilipović, Ivan and Leposavić, Gordana", year = "2014", abstract = "Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.", publisher = "Springer, New York", journal = "Biogerontology", title = "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains", pages = "486-475", number = "5", volume = "15", doi = "10.1007/s10522-014-9513-4" }
Dimitrijević, M., Stanojević, S., Vujić, V., Aleksić, I., Pilipović, I.,& Leposavić, G.. (2014). Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology Springer, New York., 15(5), 475-486. https://doi.org/10.1007/s10522-014-9513-4
Dimitrijević M, Stanojević S, Vujić V, Aleksić I, Pilipović I, Leposavić G. Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology. 2014;15(5):475-486. doi:10.1007/s10522-014-9513-4 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Vujić, Vesna, Aleksić, Iva, Pilipović, Ivan, Leposavić, Gordana, "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains" in Biogerontology, 15, no. 5 (2014):475-486, https://doi.org/10.1007/s10522-014-9513-4 . .