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dc.creatorStojković, Aleksandra
dc.creatorMaslovarić, Irina
dc.creatorKosanović, Dejana
dc.creatorVučetić, Dušan
dc.date.accessioned2021-02-18T10:42:23Z
dc.date.available2021-02-18T10:42:23Z
dc.date.issued2014
dc.identifier.issn2081-3856
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/400
dc.description.abstractA small dose of the Bordetella pertussis vaccine is used as an adjuvant for the induction of experimental autoimmune encephalomyelitis (EAE) in mice. The effects of two doses of the Pertussis vaccine on clinical signs, antibody titers, and the expression of CD4 and MHC molecules in brain tissue sections of mice with EAE were examined. EAE was induced by spinal cord homogenate in Complete Freund adjuvant (CFA) in 30 of 40 C57BL/6 mice divided in groups: EAE mice with a small adjuvant dose of the Pertussis vaccine (EAE-1), EAE mice with a human dose of the Pertussis vaccine (EAE-2), EAE mice (EAE-3). None of the mice from the EAE groups progressed to severe EAE. Five mice from the EAE-2 group were found dead on the 13th day post-immunization. A significant increase of anti-MOG (myelin oligodendrocyte glycoprotein) antibodies was detected in mice with EAE compared to non-treated mice. Myelin loss and brain tissue lesions were observed in EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A high expression of MHC-II and a mild expression of MHC-I was detected in the brains of mice with EAE. No expressions were detected in intact brains. Scattered CD4-positive cells were detected in the brains of EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A small dose of the Bordetella pertussis vaccine could maintain the developed clinical signs and histological changes in mice with EAE, while higher doses led to additional adverse effects. The expression of CD4 and MHC class I and II molecules, as well as an increase in anti-MOG antibodies could be used as markers capable of monitoring the development and progression of EAE.en
dc.publisherDe Gruyter Poland Sp Zoo, Warsaw
dc.rightsrestrictedAccess
dc.sourceTranslational Neuroscience
dc.subjectAutoimmunityen
dc.subjectEAEen
dc.subjectMHC moleculesen
dc.subjectPertussis vaccineen
dc.titlePertussis vaccine-induced experimental autoimmune encephalomyelitis in miceen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage63
dc.citation.issue1
dc.citation.other5(1): 57-63
dc.citation.rankM23
dc.citation.spage57
dc.citation.volume5
dc.identifier.doi10.2478/s13380-014-0206-x
dc.identifier.rcubconv_336
dc.identifier.scopus2-s2.0-84900348691
dc.identifier.wos000333709000007
dc.type.versionpublishedVersion


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