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dc.creatorDimitrijević, Rajna
dc.creatorIvanović, Nevena
dc.creatorMathiesen, Geir
dc.creatorPetrušić, Vladimir
dc.creatorŽivković, Irena
dc.creatorĐorđević, Brižita
dc.creatorDimitrijević, Ljiljana
dc.date.accessioned2021-02-18T10:42:14Z
dc.date.available2021-02-18T10:42:14Z
dc.date.issued2014
dc.identifier.issn0022-0299
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/398
dc.description.abstractProbiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.en
dc.publisherCambridge Univ Press, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46001/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Dairy Research
dc.subjectLactobacillus rhamnosus LA68en
dc.subjectoral administrationen
dc.subjectserum cytokinesen
dc.subjectleucocyte population shiften
dc.subjectC57BL/6 miceen
dc.titleEffects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administrationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage207
dc.citation.issue2
dc.citation.other81(2): 202-207
dc.citation.rankM21
dc.citation.spage202
dc.citation.volume81
dc.identifier.doi10.1017/S0022029914000028
dc.identifier.pmid24559976
dc.identifier.rcubconv_338
dc.identifier.scopus2-s2.0-84898818926
dc.identifier.wos000334288700009
dc.type.versionpublishedVersion


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