Strain differences in the humoral immune response to commensal bacterial antigens in rats
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2013
Authors
Kovačević-Jovanović, VesnaMiletić, Tatjana
Stanojević, Stanislava
Mitić, Katarina
Dimitrijević, Mirjana
Article (Published version)
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Show full item recordAbstract
We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked di...fferences in the immune reactivity of AO and DA rats.
Keywords:
commensal bacteria / humoral immune response / mesenteric lymph node cells / tolerance / rat strainsSource:
Acta Microbiologica et Immunologica Hungarica, 2013, 60, 3, 271-288Publisher:
- Akademiai Kiado Rt, Budapest
Funding / projects:
- Immune system plasticity during aging: Immunomodulatory capacity of oestrogens (RS-MESTD-Basic Research (BR or ON)-175050)
DOI: 10.1556/AMicr.60.2013.3.4
ISSN: 1217-8950
PubMed: 24060552
WoS: 000324913900004
Scopus: 2-s2.0-84884850671
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TorlakTY - JOUR AU - Kovačević-Jovanović, Vesna AU - Miletić, Tatjana AU - Stanojević, Stanislava AU - Mitić, Katarina AU - Dimitrijević, Mirjana PY - 2013 UR - http://intor.torlakinstitut.com/handle/123456789/386 AB - We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats. PB - Akademiai Kiado Rt, Budapest T2 - Acta Microbiologica et Immunologica Hungarica T1 - Strain differences in the humoral immune response to commensal bacterial antigens in rats EP - 288 IS - 3 SP - 271 VL - 60 DO - 10.1556/AMicr.60.2013.3.4 ER -
@article{ author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana", year = "2013", abstract = "We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.", publisher = "Akademiai Kiado Rt, Budapest", journal = "Acta Microbiologica et Immunologica Hungarica", title = "Strain differences in the humoral immune response to commensal bacterial antigens in rats", pages = "288-271", number = "3", volume = "60", doi = "10.1556/AMicr.60.2013.3.4" }
Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2013). Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica Akademiai Kiado Rt, Budapest., 60(3), 271-288. https://doi.org/10.1556/AMicr.60.2013.3.4
Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica. 2013;60(3):271-288. doi:10.1556/AMicr.60.2013.3.4 .
Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences in the humoral immune response to commensal bacterial antigens in rats" in Acta Microbiologica et Immunologica Hungarica, 60, no. 3 (2013):271-288, https://doi.org/10.1556/AMicr.60.2013.3.4 . .