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dc.creatorStein, Elisabeth
dc.creatorInić-Kanada, Aleksandra
dc.creatorBelij, Sandra
dc.creatorMontanaro, Jacqueline
dc.creatorBintner, Nora
dc.creatorSchlacher, Simone
dc.creatorMayr, Ulrike Beate
dc.creatorLubitz, Werner
dc.creatorStojanović, Marijana
dc.creatorNajdenski, Hristo
dc.creatorBarisani-Asenbauer, Talin
dc.date.accessioned2021-02-18T10:41:01Z
dc.date.available2021-02-18T10:41:01Z
dc.date.issued2013
dc.identifier.issn0146-0404
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/381
dc.description.abstractPURPOSE. For the successful topical administration of drugs or vaccines to treat ocular surface diseases, efficient and well-tolerated delivery systems/carriers for conjunctival delivery are crucial in the development of new treatment strategies. The present study investigated the efficiency of internalization of bacterial ghosts (BGs) produced from probiotic Escherichia coli Nissle 1917 (EcN) by human conjunctival epithelial (HCjE) cell line, the EcN BGs cytotoxicity for HCjE cells, and in vivo uptake of EcN BGs by conjunctival guinea pig epithelial cells. METHODS. The uptake of EcN BGs by HCjE cells was analyzed by laser scanning microscopy and flow cytometry. Immunohistochemistry was used to localize the EcN BGs in the guinea pig conjunctival tissue. Cytotoxicity of EcN BGs on HCjE cells was evaluated by measurement of LDH. RESULTS. Laser scanning microscopy and flow cytometry revealed that EcN BGs internalization by HCjE cells was time-and dose dependent. No cytotoxic effect on HCjE cells was observed after EcN BGs inoculation for 30 and 120 minutes, as well as 24 hours. In addition, the uptake of EcN BGs was detected in the conjunctival cells after in vivo administration of EcN BGs into the eye of the guinea pig. CONCLUSIONS. The findings that EcN BGs are nontoxic and effectively internalized in vitro by human and in vivo by guinea pig conjunctival cells comprise an important contribution to the future use of BGs as a system for conjunctival delivery of drugs and vaccines, either to treat or prevent ocular surface diseases.en
dc.publisherAssoc Research Vision Ophthalmology Inc, Rockville
dc.relationLaura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
dc.rightsrestrictedAccess
dc.sourceInvestigative Ophthalmology & Visual Science
dc.subjectbacterial ghostsen
dc.subjectocular surfaceen
dc.subjecthuman conjunctival cell lineen
dc.subjectdrug deliveryen
dc.subjectguinea pigen
dc.titleIn Vitro and In Vivo Uptake Study of Escherichia coli Nissle 1917 Bacterial Ghosts: Cell-Based Delivery System to Target Ocular Surface Diseasesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage6333
dc.citation.issue9
dc.citation.other54(9): 6326-6333
dc.citation.rankM21
dc.citation.spage6326
dc.citation.volume54
dc.identifier.doi10.1167/iovs.13-12044
dc.identifier.pmid23920373
dc.identifier.rcubconv_320
dc.identifier.scopus2-s2.0-84884540406
dc.identifier.wos000325169500049
dc.type.versionpublishedVersion


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