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Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic

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2013
365.pdf (729.8Kb)
Authors
Maslovarić, Irina
Vukov, Nataša
Stojković, Aleksandra
Kosanović, Dejana
Jovanova-Nešić, Katica
Article (Published version)
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Abstract
Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mic...e.

Keywords:
EAE / MOG / lymph nodes / spleen
Source:
Archives of Biological Sciences, 2013, 65, 4, 1317-1322
Publisher:
  • Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
  • Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
  • Immune system plasticity during aging: Immunomodulatory capacity of oestrogens (RS-175050)

DOI: 10.2298/ABS1304317M

ISSN: 0354-4664

WoS: 000330326600007

Scopus: 2-s2.0-84884697733
[ Google Scholar ]
3
3
URI
http://intor.torlakinstitut.com/handle/123456789/368
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Maslovarić, Irina
AU  - Vukov, Nataša
AU  - Stojković, Aleksandra
AU  - Kosanović, Dejana
AU  - Jovanova-Nešić, Katica
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/368
AB  - Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic
EP  - 1322
IS  - 4
SP  - 1317
VL  - 65
DO  - 10.2298/ABS1304317M
UR  - conv_33
ER  - 
@article{
author = "Maslovarić, Irina and Vukov, Nataša and Stojković, Aleksandra and Kosanović, Dejana and Jovanova-Nešić, Katica",
year = "2013",
abstract = "Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic",
pages = "1322-1317",
number = "4",
volume = "65",
doi = "10.2298/ABS1304317M",
url = "conv_33"
}
Maslovarić, I., Vukov, N., Stojković, A., Kosanović, D.,& Jovanova-Nešić, K.. (2013). Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(4), 1317-1322.
https://doi.org/10.2298/ABS1304317M
conv_33
Maslovarić I, Vukov N, Stojković A, Kosanović D, Jovanova-Nešić K. Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic. in Archives of Biological Sciences. 2013;65(4):1317-1322.
doi:10.2298/ABS1304317M
conv_33 .
Maslovarić, Irina, Vukov, Nataša, Stojković, Aleksandra, Kosanović, Dejana, Jovanova-Nešić, Katica, "Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic" in Archives of Biological Sciences, 65, no. 4 (2013):1317-1322,
https://doi.org/10.2298/ABS1304317M .,
conv_33 .

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