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Molecular and immunological characterization of Mus a 5 allergen from banana fruit
dc.creator | Aleksić, Ivana | |
dc.creator | Popović, Milica | |
dc.creator | Dimitrijević, Rajna | |
dc.creator | Anđelković, Uroš | |
dc.creator | Vassilopoulou, Emilia | |
dc.creator | Sinaniotis, Athanassios | |
dc.creator | Atanasković-Marković, Marina | |
dc.creator | Lindner, Buko | |
dc.creator | Petersen, Arnd | |
dc.creator | Papadopoulos, Nikolaos G. | |
dc.creator | Gavrović-Jankulović, Marija | |
dc.date.accessioned | 2021-02-18T10:39:33Z | |
dc.date.available | 2021-02-18T10:39:33Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1613-4125 | |
dc.identifier.uri | http://intor.torlakinstitut.com/handle/123456789/362 | |
dc.description.abstract | Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.rights | restrictedAccess | |
dc.source | Molecular Nutrition and Food Research | |
dc.subject | Allergen | en |
dc.subject | Banana | en |
dc.subject | Food allergy | en |
dc.subject | Glucanase | en |
dc.subject | Mus a 5 | en |
dc.title | Molecular and immunological characterization of Mus a 5 allergen from banana fruit | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 453 | |
dc.citation.issue | 3 | |
dc.citation.other | 56(3): 446-453 | |
dc.citation.rank | aM21 | |
dc.citation.spage | 446 | |
dc.citation.volume | 56 | |
dc.identifier.doi | 10.1002/mnfr.201100541 | |
dc.identifier.pmid | 22162266 | |
dc.identifier.scopus | 2-s2.0-84859181170 | |
dc.identifier.wos | 000302169000008 | |
dc.type.version | publishedVersion |