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dc.creatorGrozdanović, Milica
dc.creatorPopović, Milica
dc.creatorPolović, Natalija
dc.creatorBurazer, Lidija
dc.creatorVučković, Olga
dc.creatorAtanasković-Marković, Marina
dc.creatorLindner, Buko
dc.creatorPetersen, Arnd
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2021-02-18T10:39:17Z
dc.date.available2021-02-18T10:39:17Z
dc.date.issued2012
dc.identifier.issn0278-6915
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/358
dc.description.abstractActinidin, an abundant cysteine protease from kiwifruit, is a specific biomarker of isolated allergy to kiwifruit. This study evaluates the IgE-binding properties of biologically active and thermally inactivated actinidin. Employing two different activity assays (caseinolytic assay and zymogram with gelatin) we showed that actinidin obtained from kiwifruit extract under native conditions represents a mixture of inactive and active enzyme. The structural integrity of actinidin was confirmed by SDS-PAGE. Edman degradation, mass fingerprint and Western blot with polyclonal antibodies. Although it was capable of inducing positive skin prick test reactions, we failed to detect IgE reactivity of active actinidin in Western blot with patient sera. Thermally inactivated actinidin exhibited IgE reactivity both in vivo and in vitro, indicating that heat processed kiwifruit products may induce clinical reactivity. These findings imply that apart from the allergenic epitopes on its surface, actinidin also contains hidden epitopes inside the protein which become accessible to IgE upon thermal treatment. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceFood and Chemical Toxicology
dc.subjectAct d 1en
dc.subjectActinidinen
dc.subjectFood allergyen
dc.subjectCysteine proteaseen
dc.subjectIgE reactivityen
dc.titleEvaluation of IgE reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergyen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1018
dc.citation.issue3-4
dc.citation.other50(3-4): 1013-1018
dc.citation.rankaM21
dc.citation.spage1013
dc.citation.volume50
dc.identifier.doi10.1016/j.fct.2011.12.030
dc.identifier.pmid22227218
dc.identifier.rcubconv_290
dc.identifier.scopus2-s2.0-84856410784
dc.identifier.wos000303284600081
dc.type.versionpublishedVersion


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