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dc.creatorStenovec, Matjaz
dc.creatorMilošević, Milena
dc.creatorPetrušić, Vladimir
dc.creatorPotokar, Maja
dc.creatorStević, Zorica
dc.creatorPrebil, Mateja
dc.creatorKreft, Marko
dc.creatorTrkov, Saša
dc.creatorAnđus, Pavle
dc.creatorZorec, Robert
dc.date.accessioned2021-02-18T10:37:28Z
dc.date.available2021-02-18T10:37:28Z
dc.date.issued2011
dc.identifier.issn1748-1708
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/331
dc.description.abstractAim: We examined the effect of purified immunoglobulins G (IgG) from patients with amyotrophic lateral sclerosis (ALS) on the mobility and exocytotic release from Lysotracker-stained vesicles in cultured rat astrocytes. Methods: Time-lapse confocal images were acquired, and vesicle mobility was analysed before and after the application of ALS IgG. The vesicle counts were obtained to assess cargo exocytosis from stained organelles. Results: At rest, when mobility was monitored for 2 min in bath with Ca2+, two vesicle populations were discovered: (1) non-mobile vesicles (6.1%) with total track length (TL) lt 1 mu m, averaging at 0.33 +/- 0.01 mu m (n = 1305) and (2) mobile vesicles (93.9%) with TL gt 1 mu m, averaging at 3.03 +/- 0.01 mu m (n = 20 200). ALS IgG (0.1 mg mL(-1)) from 12 of 13 patients increased the TL of mobile vesicles by approx. 24% and maximal displacement (MD) by approx. 26% within 4 min, while the IgG from control group did not alter the vesicle mobility. The mobility enhancement by ALS IgG was reduced in extracellular solution devoid of Ca2+, indicating that ALS IgG vesicle mobility enhancement involves changes in Ca2+ homeostasis. To examine whether enhanced mobility relates to elevated Ca2+ activity, cells were stimulated by 1 mm ATP, a cytosolic Ca2+ increasing agent, in the presence (2 mm) and in the absence of extracellular Ca2+. ATP stimulation triggered an increase in TL by approx. 7% and 12% and a decrease in MD by approx. 11% and 1%, within 4 min respectively. Interestingly, none of the stimuli triggered the release of vesicle cargo. Conclusion: Amyotrophic lateral sclerosis-IgG-enhanced vesicle mobility in astrocytes engages changes in calcium homeostasis.en
dc.publisherWiley, Hoboken
dc.relationSlovenian Research Agency (ARRS)Slovenian Research Agency - Slovenia [P3 310 0381]
dc.relationEduGlia ESR
dc.relationNEUROIMAGE SSA-INCO [024600, 41005]
dc.rightsrestrictedAccess
dc.sourceActa Physiologica
dc.subjectamyotrophic lateral sclerosisen
dc.subjectastrocytesen
dc.subjectendosomesen
dc.subjectIgGen
dc.subjectlysosomesen
dc.subjectvesicle mobilityen
dc.titleAmyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage471
dc.citation.issue4
dc.citation.other203(4): 457-471
dc.citation.rankM22
dc.citation.spage457
dc.citation.volume203
dc.identifier.doi10.1111/j.1748-1716.2011.02337.x
dc.identifier.pmid21726417
dc.identifier.scopus2-s2.0-80055028971
dc.identifier.wos000296490700007
dc.type.versionpublishedVersion


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