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dc.creatorLukić, Ivana
dc.creatorDimitrijević, Ljiljana
dc.creatorDovezenski, Nebojsa
dc.creatorŽivković, Irena
dc.creatorPetrušić, Vladimir
dc.creatorMarinković, Emilija
dc.creatorInić-Kanada, Aleksandra
dc.creatorStojanović, Marijana
dc.date.accessioned2021-02-18T10:36:40Z
dc.date.available2021-02-18T10:36:40Z
dc.date.issued2011
dc.identifier.issn1570-0232
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/319
dc.description.abstractGiven an existing demand to establish a process of tetanus vaccine production in a way that allows its complete validation and standardization, this paper focuses on tetanus toxoid purification step. More precisely, we were looking at a possibility to replace the widely used ammonium-sulphate precipitation by a chromatographic method. Based on the tetanus toxin's biochemical characteristics, we have decided to examine the possibility of tetanus toxoid purification by hydrophobic chromatography, and by chromatographic techniques based on interaction with immobilized metal ions, i.e. chelating chromatography and immobilized metal affinity chromatography. We used samples obtained from differently fragmented crude tetanus toxins by formaldehyde treatment (assigned as TTd-A and TTd-B) as starting material for tetanus toxoid purification. Obtained results imply that purification of tetanus toxoid by hydrophobic chromatography represents a good alternative to ammonium-sulphate precipitation. Tetanus toxoid preparations obtained by hydrophobic chromatography were similar to those obtained by ammonium-sulphate precipitation in respect to yield, purity and immunogenicity. In addition, their immunogenicity was similar to standard tetanus toxoid preparation (NIBSC, Potters Bar, UK). Furthermore, the characteristics of crude tetanus toxin preparations had the lowest impact on the final purification product when hydrophobic chromatography was the applied method of tetanus toxoid purification. On the other hand, purifications of tetanus toxoid by chelating chromatography or immobilized metal affinity chromatography generally resulted in a very low yield due to not satisfactory tetanus toxoid binding to the column, and immunogenicity of the obtained tetanus toxoid-containing preparations was poor. (C) 2011 Elsevier BM. All rights reserved.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
dc.subjectTetanus toxoiden
dc.subjectHydrophobic chromatographyen
dc.subjectChelating chromatographyen
dc.subjectImmobilized metal affinity chromatographyen
dc.subjectAmmonium-sulphate precipitationen
dc.titleTetanus toxoid purification: Chromatographic procedures as an alternative to ammonium-sulphate precipitationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage2219
dc.citation.issue23
dc.citation.other879(23): 2213-2219
dc.citation.rankM22
dc.citation.spage2213
dc.citation.volume879
dc.identifier.doi10.1016/j.jchromb.2011.06.003
dc.identifier.pmid21741330
dc.identifier.rcubconv_269
dc.identifier.scopus2-s2.0-79960564164
dc.identifier.wos000293722800006
dc.type.versionpublishedVersion


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