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dc.creatorPopović, Milica
dc.creatorMilovanović, Mina
dc.creatorBurazer, Lidija
dc.creatorVučković, Olga
dc.creatorHoffmann-Sommergruber, Karin
dc.creatorKnulst, Andre C.
dc.creatorLindner, Buko
dc.creatorPetersen, Arnd
dc.creatorJankov, Ratko
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2021-02-18T10:35:45Z
dc.date.available2021-02-18T10:35:45Z
dc.date.issued2010
dc.identifier.issn1613-4125
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/305
dc.description.abstractKiwifruit has become a frequent cause of fruit allergy in the recent years. The molecular basis of type I hypersensitivity to kiwifruit is attributed to 11 IUIS allergens, with Act d 1, Act d 2 and Act d 5 characterized in extenso. Evaluation of the allergenic properties of Act d 4, a cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa) was performed in this study. Identity of the purified glycoprotein was determined by Edman degradation and by mass fingerprint whereby more than 90% of the primary structure of the mature kiwifruit cystatin was confirmed. Using MALDI TOF analysis, molecular masses of 10902.5 and 11055.2 Da were detected for Act d 4, respectively. Positive skin prick reactivity with Act d 4 was induced in three kiwifruit allergic patients, as well as the upregulation of CD63 and CD203c molecules in the basophile activation assay. The IgE reactivity was detected in dot blot analysis while Western blot analysis was negative using sera from six kiwifruit patients, suggesting the presence of conformational IgE epitopes on the Act d 4 molecule. As activator of effector cells in type I hypersensitivity Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsrestrictedAccess
dc.sourceMolecular Nutrition and Food Research
dc.subjectAct d 4en
dc.subjectAllergenen
dc.subjectCysteine proteinase inhibitoren
dc.subjectKiwifruiten
dc.subjectPhytocystatinen
dc.titleCysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergyen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage380
dc.citation.issue3
dc.citation.other54(3): 373-380
dc.citation.rankaM21
dc.citation.spage373
dc.citation.volume54
dc.identifier.doi10.1002/mnfr.200900035
dc.identifier.pmid19885843
dc.identifier.rcubconv_251
dc.identifier.scopus2-s2.0-77649298828
dc.identifier.wos000276063900007
dc.type.versionpublishedVersion


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