Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.
Ključne reči:
EAE / choroid plexus / GJ / Cx43 / AFM topography
Izvor:
Contemporary Challenges in Autoimmunity, 2009, 1173, 75-82
TY - JOUR
AU - Jovanova-Nešić, Katica
AU - Koruga, D.
AU - Kojić, D.
AU - Kostić, V.
AU - Rakić, L.
AU - Shoenfeld, Yehuda
PY - 2009
UR - http://intor.torlakinstitut.com/handle/123456789/291
AB - Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.
PB - Wiley-Blackwell, Malden
T2 - Contemporary Challenges in Autoimmunity
T1 - Choroid Plexus Connexin 43 Expression and Gap Junction Flexibility Are Associated with Clinical Features of Acute EAE
EP - 82
SP - 75
VL - 1173
DO - 10.1111/j.1749-6632.2009.04658.x
UR - conv_241
ER -
@article{
author = "Jovanova-Nešić, Katica and Koruga, D. and Kojić, D. and Kostić, V. and Rakić, L. and Shoenfeld, Yehuda",
year = "2009",
abstract = "Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.",
publisher = "Wiley-Blackwell, Malden",
journal = "Contemporary Challenges in Autoimmunity",
title = "Choroid Plexus Connexin 43 Expression and Gap Junction Flexibility Are Associated with Clinical Features of Acute EAE",
pages = "82-75",
volume = "1173",
doi = "10.1111/j.1749-6632.2009.04658.x",
url = "conv_241"
}
Jovanova-Nešić, K., Koruga, D., Kojić, D., Kostić, V., Rakić, L.,& Shoenfeld, Y.. (2009). Choroid Plexus Connexin 43 Expression and Gap Junction Flexibility Are Associated with Clinical Features of Acute EAE. in Contemporary Challenges in Autoimmunity
Wiley-Blackwell, Malden., 1173, 75-82.
https://doi.org/10.1111/j.1749-6632.2009.04658.x
conv_241
Jovanova-Nešić K, Koruga D, Kojić D, Kostić V, Rakić L, Shoenfeld Y. Choroid Plexus Connexin 43 Expression and Gap Junction Flexibility Are Associated with Clinical Features of Acute EAE. in Contemporary Challenges in Autoimmunity. 2009;1173:75-82.
doi:10.1111/j.1749-6632.2009.04658.x
conv_241 .
Jovanova-Nešić, Katica, Koruga, D., Kojić, D., Kostić, V., Rakić, L., Shoenfeld, Yehuda, "Choroid Plexus Connexin 43 Expression and Gap Junction Flexibility Are Associated with Clinical Features of Acute EAE" in Contemporary Challenges in Autoimmunity, 1173 (2009):75-82,
https://doi.org/10.1111/j.1749-6632.2009.04658.x .,
conv_241 .
