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Hemijske modifikacije Art v 1, glavnog alergena Artemisia vulgaris, cis-akonitilovanjem i citrakonilovanjem

dc.creatorStanić, Dragana
dc.creatorBurazer, Lidija
dc.creatorGavrović-Jankulović, Marija
dc.creatorJankov, Ratko
dc.creatorĆirković-Veličković, Tanja
dc.date.accessioned2021-02-18T10:34:29Z
dc.date.available2021-02-18T10:34:29Z
dc.date.issued2009
dc.identifier.issn0352-5139
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/286
dc.description.abstractArt v 1 is the major allergen of mugwort (Artemisia vulgaris) pollen, a significant cause of hay fever all over Europe. Specific immunotherapy is the only treatment modality for allergic disease. Application of modified allergens makes the treatment safer and more efficient. In this work, two out of three (citraconic anhydride, cis-aconitic anhydride, 2,3-dimethylmaleic anhydride) tested anhydrides were proven to be suitable for chemical modifications of allergens. Art v 1 was modified by cis-aconitylation and citraconylation in order to obtain derivatives of Art v 1 that may be suitable for further immunological testing. Acylation of Art v 1 gave derivatives (caaArt v 1 and citArt v 1) with about 80 % modified amino groups. The derivatives were in the monomeric form and had dramatically reduced pI values. Both derivatives were relatively stable at neutral pH values, while the acyl groups undergo hydrolysis under acidic conditions. Modification of allergens by cis-aconitylation and citraconylation could be a new tool for obtaining allergoids.en
dc.description.abstractArt v1 je glavni alergen polena crnog pelina (Artemisia vulgaris), značajnog uzročnika polenske groznice širom Evrope. Alergen-specifična imunoterapija je za sada jedini delotvoran način za tretiranje alergija, pri čemu primena modifikovanih alergena čini ovakav tretman bezbednijim i efikasnijim. U ovom radu, dva od tri (anhidrid cis-akonitne, citrakonske i 2,3-dimetilmaleinske kiseline) ispitivana anhidrida su se pokazala pogodnim za hemijske modifikacije alergena. Art v 1 je modifikovan cis-akonitilovanjem i citrakonilovanjem u cilju dobijanja derivata Art v 1 pogodnih za dalje imunološke testove. Acilovanjem Art v 1 dobijeni su derivati (caaArt v 1 i citArt v 1) sa oko 80 % izmodifikovanih amino grupa. Dobijeni derivati su monomerni, sa molekulskom masom sličnom nativnom Art v 1, ali sa dramatično smanjenim pI vrednostima. Oba derivata su relativno stabilna u neutralnoj, dok se u kiseloj sredini acil grupe hidrolizuju. Modifikacija alergena cis-akonitilovanjem i citrakonilovanjem može biti novi način za dobijanje alergoida.sr
dc.publisherSrpsko hemijsko društvo, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of the Serbian Chemical Society
dc.subjectallergoiden
dc.subjectmugwort pollenen
dc.subjectArt v 1en
dc.subjectchemical modificationen
dc.subjectallergen-specific immunotherapyen
dc.titleChemical modification of Art v 1, a major mugwort pollen allergen, by cis-aconitylation and citraconylationen
dc.titleHemijske modifikacije Art v 1, glavnog alergena Artemisia vulgaris, cis-akonitilovanjem i citrakonilovanjemsr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage366
dc.citation.issue4
dc.citation.other74(4): 359-366
dc.citation.rankM23
dc.citation.spage359
dc.citation.volume74
dc.identifier.doi10.2298/JSC0904359S
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/142/283.pdf
dc.identifier.rcubconv_21
dc.identifier.scopus2-s2.0-68949143451
dc.identifier.wos000266210200001
dc.type.versionpublishedVersion


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