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dc.creatorInić-Kanada, Aleksandra
dc.creatorStojanović, Marijana
dc.creatorŽivković, Irena
dc.creatorKosec, Duško
dc.creatorMićić, Mileva
dc.creatorPetrušić, Vladimir
dc.creatorŽivančević-Simonović, Snežana
dc.creatorDimitrijević, Ljiljana
dc.date.accessioned2021-02-18T10:34:17Z
dc.date.available2021-02-18T10:34:17Z
dc.date.issued2009
dc.identifier.issn1046-7408
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/283
dc.description.abstractStudies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein beta(2)-glycoprotein I (beta(2)GPI) and structure within micro-organisms or their products, might be a cause for experimental APS. Considering the heterogeneity of polyclonal antiphospholipid antibodies (aPLs), it is important to define the precise characteristics of pathogenic aPLs. To avoid the influence of polyclonality and to further analyse the connection between molecular mimicry and APS, we produced monoclonal antibodies (MAbs) against tetanus toxoid (TTd) and tested their reactivity against beta(2)GPI. In this report, we analysed the characteristics of MAb26 raised against TTd and cross-reactive with beta(2)GPI: its binding properties in various in vitro immunoassays, its specific interactions with surface epitopes expressed on apoptotic cells and its role in vivo. We have demonstrated that MAb26: (i) binds beta(2)GPI being immobilized on an appropriate surface: irradiated polystyrene plates, non-irradiated plates pre-coated with anionic phospholipids and polyvinylidene fluoride membrane; (ii) binds specifically to apoptotic but not to viable cells and the binding is beta(2)GPI-dependent; and (iii) induces a pathologic pregnancy outcome when passively injected into BALB/c mice. This study concluded that certain subpopulations of antibodies raised against TTd and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsrestrictedAccess
dc.sourceAmerican Journal of Reproductive Immunology
dc.subjectAntiphospholipid syndromeen
dc.subjectfetal lossen
dc.subjectmolecular mimicryen
dc.subjecttetanus toxoiden
dc.subjectbeta(2)-glycoprotein Ien
dc.titleMurine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicryen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage51
dc.citation.issue1
dc.citation.other61(1): 39-51
dc.citation.rankM22
dc.citation.spage39
dc.citation.volume61
dc.identifier.doi10.1111/j.1600-0897.2008.00660.x
dc.identifier.pmid19086991
dc.identifier.rcubconv_223
dc.identifier.scopus2-s2.0-57749199106
dc.identifier.wos000261636900006
dc.type.versionpublishedVersion


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