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Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry

Authorized Users Only
2009
Authors
Inić-Kanada, Aleksandra
Stojanović, Marijana
Živković, Irena
Kosec, Duško
Mićić, Mileva
Petrušić, Vladimir
Živančević-Simonović, Snežana
Dimitrijević, Ljiljana
Article (Published version)
Metadata
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Abstract
Studies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein beta(2)-glycoprotein I (beta(2)GPI) and structure within micro-organisms or their products, might be a cause for experimental APS. Considering the heterogeneity of polyclonal antiphospholipid antibodies (aPLs), it is important to define the precise characteristics of pathogenic aPLs. To avoid the influence of polyclonality and to further analyse the connection between molecular mimicry and APS, we produced monoclonal antibodies (MAbs) against tetanus toxoid (TTd) and tested their reactivity against beta(2)GPI. In this report, we analysed the characteristics of MAb26 raised against TTd and cross-reactive with beta(2)GPI: its binding properties in various in vitro immunoassays, its specific interactions with surface epitopes expressed on apoptotic cells and its role in vivo. We have demonstrated that MAb26: (i) binds beta(2)GPI being immobilized on an appropriate surface: i...rradiated polystyrene plates, non-irradiated plates pre-coated with anionic phospholipids and polyvinylidene fluoride membrane; (ii) binds specifically to apoptotic but not to viable cells and the binding is beta(2)GPI-dependent; and (iii) induces a pathologic pregnancy outcome when passively injected into BALB/c mice. This study concluded that certain subpopulations of antibodies raised against TTd and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential.

Keywords:
Antiphospholipid syndrome / fetal loss / molecular mimicry / tetanus toxoid / beta(2)-glycoprotein I
Source:
American Journal of Reproductive Immunology, 2009, 61, 1, 39-51
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima (RS-142020)

DOI: 10.1111/j.1600-0897.2008.00660.x

ISSN: 1046-7408

PubMed: 19086991

WoS: 000261636900006

Scopus: 2-s2.0-57749199106
[ Google Scholar ]
20
17
URI
http://intor.torlakinstitut.com/handle/123456789/283
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Živković, Irena
AU  - Kosec, Duško
AU  - Mićić, Mileva
AU  - Petrušić, Vladimir
AU  - Živančević-Simonović, Snežana
AU  - Dimitrijević, Ljiljana
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/283
AB  - Studies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein beta(2)-glycoprotein I (beta(2)GPI) and structure within micro-organisms or their products, might be a cause for experimental APS. Considering the heterogeneity of polyclonal antiphospholipid antibodies (aPLs), it is important to define the precise characteristics of pathogenic aPLs. To avoid the influence of polyclonality and to further analyse the connection between molecular mimicry and APS, we produced monoclonal antibodies (MAbs) against tetanus toxoid (TTd) and tested their reactivity against beta(2)GPI. In this report, we analysed the characteristics of MAb26 raised against TTd and cross-reactive with beta(2)GPI: its binding properties in various in vitro immunoassays, its specific interactions with surface epitopes expressed on apoptotic cells and its role in vivo. We have demonstrated that MAb26: (i) binds beta(2)GPI being immobilized on an appropriate surface: irradiated polystyrene plates, non-irradiated plates pre-coated with anionic phospholipids and polyvinylidene fluoride membrane; (ii) binds specifically to apoptotic but not to viable cells and the binding is beta(2)GPI-dependent; and (iii) induces a pathologic pregnancy outcome when passively injected into BALB/c mice. This study concluded that certain subpopulations of antibodies raised against TTd and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry
EP  - 51
IS  - 1
SP  - 39
VL  - 61
DO  - 10.1111/j.1600-0897.2008.00660.x
UR  - conv_223
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Živković, Irena and Kosec, Duško and Mićić, Mileva and Petrušić, Vladimir and Živančević-Simonović, Snežana and Dimitrijević, Ljiljana",
year = "2009",
abstract = "Studies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein beta(2)-glycoprotein I (beta(2)GPI) and structure within micro-organisms or their products, might be a cause for experimental APS. Considering the heterogeneity of polyclonal antiphospholipid antibodies (aPLs), it is important to define the precise characteristics of pathogenic aPLs. To avoid the influence of polyclonality and to further analyse the connection between molecular mimicry and APS, we produced monoclonal antibodies (MAbs) against tetanus toxoid (TTd) and tested their reactivity against beta(2)GPI. In this report, we analysed the characteristics of MAb26 raised against TTd and cross-reactive with beta(2)GPI: its binding properties in various in vitro immunoassays, its specific interactions with surface epitopes expressed on apoptotic cells and its role in vivo. We have demonstrated that MAb26: (i) binds beta(2)GPI being immobilized on an appropriate surface: irradiated polystyrene plates, non-irradiated plates pre-coated with anionic phospholipids and polyvinylidene fluoride membrane; (ii) binds specifically to apoptotic but not to viable cells and the binding is beta(2)GPI-dependent; and (iii) induces a pathologic pregnancy outcome when passively injected into BALB/c mice. This study concluded that certain subpopulations of antibodies raised against TTd and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry",
pages = "51-39",
number = "1",
volume = "61",
doi = "10.1111/j.1600-0897.2008.00660.x",
url = "conv_223"
}
Inić-Kanada, A., Stojanović, M., Živković, I., Kosec, D., Mićić, M., Petrušić, V., Živančević-Simonović, S.,& Dimitrijević, L.. (2009). Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry. in American Journal of Reproductive Immunology
Wiley, Hoboken., 61(1), 39-51.
https://doi.org/10.1111/j.1600-0897.2008.00660.x
conv_223
Inić-Kanada A, Stojanović M, Živković I, Kosec D, Mićić M, Petrušić V, Živančević-Simonović S, Dimitrijević L. Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry. in American Journal of Reproductive Immunology. 2009;61(1):39-51.
doi:10.1111/j.1600-0897.2008.00660.x
conv_223 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Živković, Irena, Kosec, Duško, Mićić, Mileva, Petrušić, Vladimir, Živančević-Simonović, Snežana, Dimitrijević, Ljiljana, "Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with beta(2)-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry" in American Journal of Reproductive Immunology, 61, no. 1 (2009):39-51,
https://doi.org/10.1111/j.1600-0897.2008.00660.x .,
conv_223 .

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