Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14
Само за регистроване кориснике
2007
Аутори
Petrović-Đergović, Danica M.Rakin, Ana
Dimitrijević, Ljiljana
Ristovski, Jasmina S.
Kuštrimović, Nataša
Mićić, Mileva
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The role of somatostatin on inhibition of both normal and tumor cell cycle, secretion of endocrine and exocrine cells, as well as induction apoptosis is well documented. However, its effect on T cell development and thymic structure is not fully clarified. In order to investigate the influence of somatostatin in vivo on the thymus structure and T cell development, the young adult Albino Oxford male rats were intracerebroventriculary treated with somatostatin-14. We examined the thymus compartments and its cellularity, through assessment of morphometric parameters by stereological method, and the relation between thymocytes subpopulations, over expression of CD4, CD8 and T-cell receptor (TCR) alpha beta by flow cytometry. Additionally, we also determined the body and thymus weight of the rats, during the first three months of life, to define the time of SRIH-14 application. A decrease of relative thymus weight from the fourth weeks of postnatal life, and an unchanged relative thymus wei...ght obtained in treated group indicates that SRIH-14 in young adult rats inhibits growth of whole organism, not only thymus. The changes in the absolute number and numerical density of cortical thymocytes indicate that SRIH-14 alters the true lymphoid tissue. SRIH-14 changes relation between thymocyte subsets, increase number of CD4(-)CD8(-)TCR alpha beta(-) and CD4(-)CD8(+)TCR alpha beta(hi) phi thymocyte subsets as well as the CD4(-)CD8(-)TCR alpha beta(low/hi) thymocytes, while decrease number of CD4+CD8+ TCR alpha beta(low/hi) thymocyte subsets. These results indicate that somatostatin-14 is Dot involved in the control of the physiologic involution of the thymus, although induces thymic weight loss through the reduction of true lymphoid tissue. In addition, changes in frequency of thymocyte subpopulations, especially immature cells, indicate that SRIH-14 modulates thymocytes development and maturation. (C) 2007 Elsevier Ltd. All rights reserved.
Кључне речи:
rat / thymus / T cells development / Somatostatin / morphometryИзвор:
Neuropeptides, 2007, 41, 6, 485-493Издавач:
- Churchill Livingstone, Edinburgh
Финансирање / пројекти:
- Неуроендокрина модулација имунског одговора: улога симпато-адреномедуларног система (RS-MESTD-MPN2006-2010-145049)
DOI: 10.1016/j.npep.2007.06.003
ISSN: 0143-4179
PubMed: 17761280
WoS: 000252138100013
Scopus: 2-s2.0-36148994326
Институција/група
TorlakTY - JOUR AU - Petrović-Đergović, Danica M. AU - Rakin, Ana AU - Dimitrijević, Ljiljana AU - Ristovski, Jasmina S. AU - Kuštrimović, Nataša AU - Mićić, Mileva PY - 2007 UR - http://intor.torlakinstitut.com/handle/123456789/241 AB - The role of somatostatin on inhibition of both normal and tumor cell cycle, secretion of endocrine and exocrine cells, as well as induction apoptosis is well documented. However, its effect on T cell development and thymic structure is not fully clarified. In order to investigate the influence of somatostatin in vivo on the thymus structure and T cell development, the young adult Albino Oxford male rats were intracerebroventriculary treated with somatostatin-14. We examined the thymus compartments and its cellularity, through assessment of morphometric parameters by stereological method, and the relation between thymocytes subpopulations, over expression of CD4, CD8 and T-cell receptor (TCR) alpha beta by flow cytometry. Additionally, we also determined the body and thymus weight of the rats, during the first three months of life, to define the time of SRIH-14 application. A decrease of relative thymus weight from the fourth weeks of postnatal life, and an unchanged relative thymus weight obtained in treated group indicates that SRIH-14 in young adult rats inhibits growth of whole organism, not only thymus. The changes in the absolute number and numerical density of cortical thymocytes indicate that SRIH-14 alters the true lymphoid tissue. SRIH-14 changes relation between thymocyte subsets, increase number of CD4(-)CD8(-)TCR alpha beta(-) and CD4(-)CD8(+)TCR alpha beta(hi) phi thymocyte subsets as well as the CD4(-)CD8(-)TCR alpha beta(low/hi) thymocytes, while decrease number of CD4+CD8+ TCR alpha beta(low/hi) thymocyte subsets. These results indicate that somatostatin-14 is Dot involved in the control of the physiologic involution of the thymus, although induces thymic weight loss through the reduction of true lymphoid tissue. In addition, changes in frequency of thymocyte subpopulations, especially immature cells, indicate that SRIH-14 modulates thymocytes development and maturation. (C) 2007 Elsevier Ltd. All rights reserved. PB - Churchill Livingstone, Edinburgh T2 - Neuropeptides T1 - Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14 EP - 493 IS - 6 SP - 485 VL - 41 DO - 10.1016/j.npep.2007.06.003 ER -
@article{ author = "Petrović-Đergović, Danica M. and Rakin, Ana and Dimitrijević, Ljiljana and Ristovski, Jasmina S. and Kuštrimović, Nataša and Mićić, Mileva", year = "2007", abstract = "The role of somatostatin on inhibition of both normal and tumor cell cycle, secretion of endocrine and exocrine cells, as well as induction apoptosis is well documented. However, its effect on T cell development and thymic structure is not fully clarified. In order to investigate the influence of somatostatin in vivo on the thymus structure and T cell development, the young adult Albino Oxford male rats were intracerebroventriculary treated with somatostatin-14. We examined the thymus compartments and its cellularity, through assessment of morphometric parameters by stereological method, and the relation between thymocytes subpopulations, over expression of CD4, CD8 and T-cell receptor (TCR) alpha beta by flow cytometry. Additionally, we also determined the body and thymus weight of the rats, during the first three months of life, to define the time of SRIH-14 application. A decrease of relative thymus weight from the fourth weeks of postnatal life, and an unchanged relative thymus weight obtained in treated group indicates that SRIH-14 in young adult rats inhibits growth of whole organism, not only thymus. The changes in the absolute number and numerical density of cortical thymocytes indicate that SRIH-14 alters the true lymphoid tissue. SRIH-14 changes relation between thymocyte subsets, increase number of CD4(-)CD8(-)TCR alpha beta(-) and CD4(-)CD8(+)TCR alpha beta(hi) phi thymocyte subsets as well as the CD4(-)CD8(-)TCR alpha beta(low/hi) thymocytes, while decrease number of CD4+CD8+ TCR alpha beta(low/hi) thymocyte subsets. These results indicate that somatostatin-14 is Dot involved in the control of the physiologic involution of the thymus, although induces thymic weight loss through the reduction of true lymphoid tissue. In addition, changes in frequency of thymocyte subpopulations, especially immature cells, indicate that SRIH-14 modulates thymocytes development and maturation. (C) 2007 Elsevier Ltd. All rights reserved.", publisher = "Churchill Livingstone, Edinburgh", journal = "Neuropeptides", title = "Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14", pages = "493-485", number = "6", volume = "41", doi = "10.1016/j.npep.2007.06.003" }
Petrović-Đergović, D. M., Rakin, A., Dimitrijević, L., Ristovski, J. S., Kuštrimović, N.,& Mićić, M.. (2007). Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14. in Neuropeptides Churchill Livingstone, Edinburgh., 41(6), 485-493. https://doi.org/10.1016/j.npep.2007.06.003
Petrović-Đergović DM, Rakin A, Dimitrijević L, Ristovski JS, Kuštrimović N, Mićić M. Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14. in Neuropeptides. 2007;41(6):485-493. doi:10.1016/j.npep.2007.06.003 .
Petrović-Đergović, Danica M., Rakin, Ana, Dimitrijević, Ljiljana, Ristovski, Jasmina S., Kuštrimović, Nataša, Mićić, Mileva, "Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14" in Neuropeptides, 41, no. 6 (2007):485-493, https://doi.org/10.1016/j.npep.2007.06.003 . .