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dc.creatorŽivančević-Simonović, Snežana
dc.creatorStojanović, Marijana
dc.creatorInić-Kanada, Aleksandra
dc.creatorŽivković, Irena
dc.creatorOdrljin, Tatjana
dc.creatorDimitrijević, Ljiljana
dc.date.accessioned2021-02-18T10:29:26Z
dc.date.available2021-02-18T10:29:26Z
dc.date.issued2006
dc.identifier.issn0258-851X
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/215
dc.description.abstractBackground: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation.en
dc.publisherInt Inst Anticancer Research, Athens
dc.rightsrestrictedAccess
dc.sourceIn Vivo
dc.subjectnetworken
dc.subjectAb1sen
dc.subjectAb2sen
dc.subjectpolyclonal activationen
dc.titleA high dose of an idiotype generates high levels of Ab2sen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage627
dc.citation.issue5
dc.citation.other20(5): 621-627
dc.citation.rankM23
dc.citation.spage621
dc.citation.volume20
dc.identifier.pmid17091769
dc.identifier.rcubconv_185
dc.identifier.scopus2-s2.0-33750589684
dc.identifier.wos000241835400008
dc.type.versionpublishedVersion


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