A high dose of an idiotype generates high levels of Ab2s
Samo za registrovane korisnike
2006
Autori
Živančević-Simonović, SnežanaStojanović, Marijana
Inić-Kanada, Aleksandra
Živković, Irena
Odrljin, Tatjana
Dimitrijević, Ljiljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polycl...onal lymphocyte activation.
Ključne reči:
network / Ab1s / Ab2s / polyclonal activationIzvor:
In Vivo, 2006, 20, 5, 621-627Izdavač:
- Int Inst Anticancer Research, Athens
Finansiranje / projekti:
- Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima (RS-MESTD-MPN2006-2010-142020)
- Imnoregulatorne interakcije u tumorskim i autoimunskim fenomenima (RS-MESTD-MPN2006-2010-145065)
Institucija/grupa
TorlakTY - JOUR AU - Živančević-Simonović, Snežana AU - Stojanović, Marijana AU - Inić-Kanada, Aleksandra AU - Živković, Irena AU - Odrljin, Tatjana AU - Dimitrijević, Ljiljana PY - 2006 UR - http://intor.torlakinstitut.com/handle/123456789/215 AB - Background: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation. PB - Int Inst Anticancer Research, Athens T2 - In Vivo T1 - A high dose of an idiotype generates high levels of Ab2s EP - 627 IS - 5 SP - 621 VL - 20 UR - https://hdl.handle.net/21.15107/rcub_intor_215 ER -
@article{ author = "Živančević-Simonović, Snežana and Stojanović, Marijana and Inić-Kanada, Aleksandra and Živković, Irena and Odrljin, Tatjana and Dimitrijević, Ljiljana", year = "2006", abstract = "Background: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation.", publisher = "Int Inst Anticancer Research, Athens", journal = "In Vivo", title = "A high dose of an idiotype generates high levels of Ab2s", pages = "627-621", number = "5", volume = "20", url = "https://hdl.handle.net/21.15107/rcub_intor_215" }
Živančević-Simonović, S., Stojanović, M., Inić-Kanada, A., Živković, I., Odrljin, T.,& Dimitrijević, L.. (2006). A high dose of an idiotype generates high levels of Ab2s. in In Vivo Int Inst Anticancer Research, Athens., 20(5), 621-627. https://hdl.handle.net/21.15107/rcub_intor_215
Živančević-Simonović S, Stojanović M, Inić-Kanada A, Živković I, Odrljin T, Dimitrijević L. A high dose of an idiotype generates high levels of Ab2s. in In Vivo. 2006;20(5):621-627. https://hdl.handle.net/21.15107/rcub_intor_215 .
Živančević-Simonović, Snežana, Stojanović, Marijana, Inić-Kanada, Aleksandra, Živković, Irena, Odrljin, Tatjana, Dimitrijević, Ljiljana, "A high dose of an idiotype generates high levels of Ab2s" in In Vivo, 20, no. 5 (2006):621-627, https://hdl.handle.net/21.15107/rcub_intor_215 .