Background: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polycl...onal lymphocyte activation.
TY - JOUR
AU - Živančević-Simonović, Snežana
AU - Stojanović, Marijana
AU - Inić-Kanada, Aleksandra
AU - Živković, Irena
AU - Odrljin, Tatjana
AU - Dimitrijević, Ljiljana
PY - 2006
UR - http://intor.torlakinstitut.com/handle/123456789/215
AB - Background: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation.
PB - Int Inst Anticancer Research, Athens
T2 - In Vivo
T1 - A high dose of an idiotype generates high levels of Ab2s
EP - 627
IS - 5
SP - 621
VL - 20
UR - conv_185
ER -
@article{
author = "Živančević-Simonović, Snežana and Stojanović, Marijana and Inić-Kanada, Aleksandra and Živković, Irena and Odrljin, Tatjana and Dimitrijević, Ljiljana",
year = "2006",
abstract = "Background: Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation. Materials and Methods: Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence. Results: In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of non-noimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome Q not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype. Conclusion: These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation.",
publisher = "Int Inst Anticancer Research, Athens",
journal = "In Vivo",
title = "A high dose of an idiotype generates high levels of Ab2s",
pages = "627-621",
number = "5",
volume = "20",
url = "conv_185"
}
Živančević-Simonović, S., Stojanović, M., Inić-Kanada, A., Živković, I., Odrljin, T.,& Dimitrijević, L.. (2006). A high dose of an idiotype generates high levels of Ab2s. in In Vivo
Int Inst Anticancer Research, Athens., 20(5), 621-627.
conv_185
Živančević-Simonović S, Stojanović M, Inić-Kanada A, Živković I, Odrljin T, Dimitrijević L. A high dose of an idiotype generates high levels of Ab2s. in In Vivo. 2006;20(5):621-627.
conv_185 .
Živančević-Simonović, Snežana, Stojanović, Marijana, Inić-Kanada, Aleksandra, Živković, Irena, Odrljin, Tatjana, Dimitrijević, Ljiljana, "A high dose of an idiotype generates high levels of Ab2s" in In Vivo, 20, no. 5 (2006):621-627,
conv_185 .
