Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats
Authors
Miletić, TatjanaKovačević-Jovanović, Vesna
Stanojević, Stanislava
Vujić, Vesna
Kosec, Duško
Mitić, Katarina
Dimitrijević, Mirjana
Article (Published version)
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Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in ...sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71.
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Scandinavian Journal of Immunology, 2006, 64, 6, 623-632Publisher:
- Wiley, Hoboken
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DOI: 10.1111/j.1365-3083.2006.01852.x
ISSN: 0300-9475
PubMed: 17083618
WoS: 000241776300006
Scopus: 2-s2.0-33750709209
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TorlakTY - JOUR AU - Miletić, Tatjana AU - Kovačević-Jovanović, Vesna AU - Stanojević, Stanislava AU - Vujić, Vesna AU - Kosec, Duško AU - Mitić, Katarina AU - Dimitrijević, Mirjana PY - 2006 UR - http://intor.torlakinstitut.com/handle/123456789/213 AB - Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71. PB - Wiley, Hoboken T2 - Scandinavian Journal of Immunology T1 - Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats EP - 632 IS - 6 SP - 623 VL - 64 DO - 10.1111/j.1365-3083.2006.01852.x ER -
@article{ author = "Miletić, Tatjana and Kovačević-Jovanović, Vesna and Stanojević, Stanislava and Vujić, Vesna and Kosec, Duško and Mitić, Katarina and Dimitrijević, Mirjana", year = "2006", abstract = "Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71.", publisher = "Wiley, Hoboken", journal = "Scandinavian Journal of Immunology", title = "Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats", pages = "632-623", number = "6", volume = "64", doi = "10.1111/j.1365-3083.2006.01852.x" }
Miletić, T., Kovačević-Jovanović, V., Stanojević, S., Vujić, V., Kosec, D., Mitić, K.,& Dimitrijević, M.. (2006). Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats. in Scandinavian Journal of Immunology Wiley, Hoboken., 64(6), 623-632. https://doi.org/10.1111/j.1365-3083.2006.01852.x
Miletić T, Kovačević-Jovanović V, Stanojević S, Vujić V, Kosec D, Mitić K, Dimitrijević M. Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats. in Scandinavian Journal of Immunology. 2006;64(6):623-632. doi:10.1111/j.1365-3083.2006.01852.x .
Miletić, Tatjana, Kovačević-Jovanović, Vesna, Stanojević, Stanislava, Vujić, Vesna, Kosec, Duško, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats" in Scandinavian Journal of Immunology, 64, no. 6 (2006):623-632, https://doi.org/10.1111/j.1365-3083.2006.01852.x . .